2p35: Difference between revisions

Revision as of 12:14, 4 May 2008

Crystal structure of trans-aconitate methyltransferase from Agrobacterium tumefaciens

OverviewOverview

We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.

About this StructureAbout this Structure

2P35 is a Single protein structure of sequence from Agrobacterium tumefaciens str. c58. Full crystallographic information is available from OCA.

ReferenceReference

In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4. PMID:17982461 Page seeded by OCA on Sun May 4 12:14:48 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2p35.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2p35 |SIZE=350|CAPTION= <scene name='initialview01'>2p35</scene>, resolution 1.95&Aring;
+The line below this paragraph, containing "STRUCTURE_2p35", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Trans-aconitate_2-methyltransferase Trans-aconitate 2-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.144 2.1.1.144] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= tam, Atu0870, AGR_C_1589 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=176299 Agrobacterium tumefaciens str. C58])
+-->
-|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam08241 Methyltransf_11], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG4106 Tam]</span>
+{{STRUCTURE_2p35|  PDB=2p35  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2p35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p35 OCA], [http://www.ebi.ac.uk/pdbsum/2p35 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2p35 RCSB]</span>
-}}
 '''Crystal structure of trans-aconitate methyltransferase from Agrobacterium tumefaciens'''
@@ Line 33: / Line 30: @@
 [[Category: Xu, X.]]
 [[Category: Zheng, H.]]
-[[Category: agrobacterium tumefacien]]
+[[Category: Agrobacterium tumefacien]]
-[[Category: mcsg]]
+[[Category: Mcsg]]
-[[Category: midwest center for structural genomic]]
+[[Category: Midwest center for structural genomic]]
-[[Category: protein structure initiative]]
+[[Category: Protein structure initiative]]
-[[Category: psi-2]]
+[[Category: Psi-2]]
-[[Category: sam dependent methyltransferase]]
+[[Category: Sam dependent methyltransferase]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
-[[Category: trans-aconitate methyltransferase]]
+[[Category: Trans-aconitate methyltransferase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 12:14:48 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:28:07 2008''