2osl: Difference between revisions

Revision as of 11:34, 4 May 2008

Structural annotation:
Resources:	InterPro : Ipr003006, Ipr003597, Ipr013783, Ipr007110, Ipr013106, Ipr003596 Pfam : PF07686, PF07654 UniProt : Q6PJA4, Q6PIH7, P11836

Evolutionary conservation:

Toggle Conservation Colors:

Rows = identical sequences:

Further Information:

Caveats,

Explanation,
Complete Results at ConSurfDB

Resources:

FirstGlance, OCA, RCSB, PDBsum

Coordinates:

save as pdb, mmCIF, xml

Crystal structure of Rituximab Fab in complex with an epitope peptide

OverviewOverview

Rituximab is a widely used monoclonal antibody drug for treating certain lymphomas and autoimmune diseases. To understand the molecular mechanism of recognition of human CD20 by Rituximab, we determined the crystal structure of the Rituximab Fab in complex with a synthesized peptide comprising the CD20 epitope (residues 163-187) at 2.6-A resolution. The combining site of the Fab consists of four complementarity determining regions that form a large, deep pocket to accommodate the epitope peptide. The bound peptide assumes a unique cyclic conformation that is constrained by a disulfide bond and a rigid proline residue (Pro(172)). The (170)ANPS(173) motif of CD20 is deeply embedded into the pocket on the antibody surface and plays an essential role in the recognition and binding of Rituximab. The antigen-antibody interactions involve both hydrogen bonds and van der Waals contacts and display a high degree of structural and chemical complementarity. These results provide a molecular basis for the specific recognition of CD20 by Rituximab as well as valuable information for development of improved antibody drugs with better specificity and higher affinity.

About this StructureAbout this Structure

2OSL is a Single protein structure of sequence from Homo sapiens, mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for recognition of CD20 by therapeutic antibody Rituximab., Du J, Wang H, Zhong C, Peng B, Zhang M, Li B, Huo S, Guo Y, Ding J, J Biol Chem. 2007 May 18;282(20):15073-80. Epub 2007 Mar 29. PMID:17395584 Page seeded by OCA on Sun May 4 11:34:28 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2osl.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2osl |SIZE=350|CAPTION= <scene name='initialview01'>2osl</scene>, resolution 2.600&Aring;
+The line below this paragraph, containing "STRUCTURE_2osl", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2osl|  PDB=2osl  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2osl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2osl OCA], [http://www.ebi.ac.uk/pdbsum/2osl PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2osl RCSB]</span>
-}}
 '''Crystal structure of Rituximab Fab in complex with an epitope peptide'''
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 [[Category: Du, J.]]
 [[Category: Zhong, C.]]
-[[Category: chimeric antibody]]
+[[Category: Chimeric antibody]]
-[[Category: fab-peptide complex]]
+[[Category: Fab-peptide complex]]
-[[Category: rituximab]]
+[[Category: Rituximab]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 11:34:28 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:22:31 2008''