2oqn: Difference between revisions

Revision as of 11:28, 4 May 2008

High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths

OverviewOverview

Crystal cryocooling is usually employed to reduce radiation damage during X-ray crystallography. Recently, a high-pressure cryocooling method has been developed which results in excellent diffraction-quality crystals without the use of penetrative cryoprotectants. Three new developments of the method are presented here: (i) Xe-He high-pressure cryocooling for Xe SAD phasing, (ii) native sulfur SAD phasing and (iii) successful cryopreservation of crystals in thick-walled capillaries without additional cryoprotectants other than the native mother liquor. These developments may be useful for structural solution of proteins without the need for selenomethionine incorporation and for high-throughput protein crystallography.

About this StructureAbout this Structure

2OQN is a Single protein structure of sequence from Thaumatococcus daniellii. Full crystallographic information is available from OCA.

ReferenceReference

High-pressure cryocooling for capillary sample cryoprotection and diffraction phasing at long wavelengths., Kim CU, Hao Q, Gruner SM, Acta Crystallogr D Biol Crystallogr. 2007 May;63(Pt 5):653-9. Epub 2007, Apr 21. PMID:17452791 Page seeded by OCA on Sun May 4 11:28:10 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2oqn.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2oqn |SIZE=350|CAPTION= <scene name='initialview01'>2oqn</scene>, resolution 1.90&Aring;
+The line below this paragraph, containing "STRUCTURE_2oqn", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=TAR:D(-)-TARTARIC+ACID'>TAR</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2oqn|  PDB=2oqn  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2oqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oqn OCA], [http://www.ebi.ac.uk/pdbsum/2oqn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2oqn RCSB]</span>
-}}
 '''High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths'''
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 [[Category: Hao, Q.]]
 [[Category: Kim, C U.]]
-[[Category: capillary cryoprotection]]
+[[Category: Capillary cryoprotection]]
-[[Category: high pressure cryocooling]]
+[[Category: High pressure cryocooling]]
-[[Category: sulfur sad phasing]]
+[[Category: Sulfur sad phasing]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 11:28:10 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:21:43 2008''