2oq9: Difference between revisions
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'''Structure of the non-canonical Mcol5 of Hydra nematocysts''' | '''Structure of the non-canonical Mcol5 of Hydra nematocysts''' | ||
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==About this Structure== | ==About this Structure== | ||
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OQ9 OCA]. | |||
==Reference== | ==Reference== | ||
Minicollagen-15, a novel minicollagen isolated from Hydra, forms tubule structures in nematocysts., Adamczyk P, Meier S, Gross T, Hobmayer B, Grzesiek S, Bachinger HP, Holstein TW, Ozbek S, J Mol Biol. 2008 Feb 29;376(4):1008-20. Epub 2007 Dec 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18206162 18206162] | Minicollagen-15, a novel minicollagen isolated from Hydra, forms tubule structures in nematocysts., Adamczyk P, Meier S, Gross T, Hobmayer B, Grzesiek S, Bachinger HP, Holstein TW, Ozbek S, J Mol Biol. 2008 Feb 29;376(4):1008-20. Epub 2007 Dec 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18206162 18206162] | ||
[[Category: Grzesiek, S.]] | [[Category: Grzesiek, S.]] | ||
[[Category: Meier, S.]] | [[Category: Meier, S.]] | ||
[[Category: Oezbek, S.]] | [[Category: Oezbek, S.]] | ||
[[Category: | [[Category: Cis-proline]] | ||
[[Category: | [[Category: Cysteine-rich domain]] | ||
[[Category: | [[Category: Disulfide]] | ||
[[Category: | [[Category: Nematocyst]] | ||
[[Category: | [[Category: Non-canonical crd]] | ||
[[Category: | [[Category: Structural protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:26:25 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 11:26, 4 May 2008
Structure of the non-canonical Mcol5 of Hydra nematocysts
OverviewOverview
Minicollagens constitute a family of unusually short collagen molecules isolated from cnidarians. They are restricted to the nematocyst, a cylindrical explosive organelle serving in defense and capture of prey. The nematocyst capsule contains a long tubule inside of its matrix, which is expelled and everted during an ultrafast discharge process. Here, we report the cloning and characterization of a novel minicollagen in Hydra, designated minicollagen-15 (NCol-15). NCol-15, like NCol-3 and NCol-4, shows deviations from the canonical cysteine pattern in its terminal cysteine-rich domains (CRDs). Minicollagens share common domain architectures with a central collagen sequence flanked by polyproline stretches and short N- and C-terminal CRDs. The CRDs are involved in the formation of a highly resistant cysteine network, which constitutes the basic structure of the nematocyst capsule. Unlike NCol-1, which is part of the capsule wall, NCol-15 is localized to tubules, arguing for a functional differentiation of minicollagens within the nematocyst architecture. NMR analysis of the altered C-terminal CRD of NCol-15 showed a novel disulfide-linked structure within the cysteine-containing region exhibiting similar folding kinetics and stability as the canonical CRDs. Our data provide evidence for evolutionary diversification among minicollagens, which probably facilitated alterations in the morphology of the nematocyst wall and tubule.
About this StructureAbout this Structure
Full crystallographic information is available from OCA.
ReferenceReference
Minicollagen-15, a novel minicollagen isolated from Hydra, forms tubule structures in nematocysts., Adamczyk P, Meier S, Gross T, Hobmayer B, Grzesiek S, Bachinger HP, Holstein TW, Ozbek S, J Mol Biol. 2008 Feb 29;376(4):1008-20. Epub 2007 Dec 5. PMID:18206162 Page seeded by OCA on Sun May 4 11:26:25 2008