2oi6: Difference between revisions

Revision as of 10:58, 4 May 2008

E. coli GlmU- Complex with UDP-GlcNAc, CoA and GlcN-1-PO4

OverviewOverview

The biosynthesis of UDP-GlcNAc in bacteria is carried out by GlmU, an essential bifunctional uridyltransferase that catalyzes the CoA-dependent acetylation of GlcN-1-PO(4) to form GlcNAc-1-PO(4) and its subsequent condensation with UTP to form pyrophosphate and UDP-GlcNAc. As a metabolite, UDP-GlcNAc is situated at a branch point leading to the biosynthesis of lipopolysaccharide and peptidoglycan. Consequently, GlmU is regarded as an important target for potential antibacterial agents. The crystal structure of the Escherichia coli GlmU acetyltransferase active site has been determined in complexes with acetyl-CoA, CoA/GlcN-1-PO(4), and desulpho-CoA/GlcNAc-1-PO(4). These structures reveal the enzyme groups responsible for binding the substrates. A superposition of these complex structures suggests that the 2-amino group of GlcN-1-PO(4) is positioned in proximity to the acetyl-CoA to facilitate direct attack on its thioester by a ternary complex mechanism.

About this StructureAbout this Structure

2OI6 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Structure of the E. coli bifunctional GlmU acetyltransferase active site with substrates and products., Olsen LR, Vetting MW, Roderick SL, Protein Sci. 2007 Jun;16(6):1230-5. Epub 2007 May 1. PMID:17473010 Page seeded by OCA on Sun May 4 10:58:32 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2oi6.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2oi6 |SIZE=350|CAPTION= <scene name='initialview01'>2oi6</scene>, resolution 2.20&Aring;
+The line below this paragraph, containing "STRUCTURE_2oi6", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=GP1:GLUCOSAMINE+1-PHOSPHATE'>GP1</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= glmU ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+-->
-|DOMAIN=
+{{STRUCTURE_2oi6|  PDB=2oi6  |  SCENE=  }}
-|RELATEDENTRY=[[1hv9|1HV9]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2oi6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oi6 OCA], [http://www.ebi.ac.uk/pdbsum/2oi6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2oi6 RCSB]</span>
-}}
 '''E. coli GlmU- Complex with UDP-GlcNAc, CoA and GlcN-1-PO4'''
@@ Line 28: / Line 25: @@
 [[Category: Roderick, S L.]]
 [[Category: Vetting, M W.]]
-[[Category: left-handed beta helix]]
+[[Category: Left-handed beta helix]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 10:58:32 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:18:14 2008''