2obt: Difference between revisions

Revision as of 10:34, 4 May 2008

Crystal Structures of P Domain of Norovirus VA387 in Complex with Blood Group Trisaccharides type B

OverviewOverview

Noroviruses are one of the major causes of nonbacterial gastroenteritis epidemics in humans. Recent studies on norovirus receptors show that different noroviruses recognize different human histo-blood group antigens (HBGAs), and eight receptor binding patterns of noroviruses have been identified. The P domain of the norovirus capsids is directly involved in this recognition. To determine the precise locations and receptor binding modes of HBGA carbohydrates on the viral capsids, a recombinant P protein of a GII-4 strain norovirus, VA387, was cocrystallized with synthetic type A or B trisaccharides. Based on complex crystal structures observed at a 2.0-A resolution, we demonstrated that the receptor binding site lies at the outermost end of the P domain and forms an extensive hydrogen-bonding network with the saccharide ligand. The A and B trisaccharides display similar binding modes, and the common fucose ring plays a key role in this interaction. The extensive interface between the two protomers in a P dimer also plays a crucial role in the formation of the receptor binding interface.

About this StructureAbout this Structure

2OBT is a Single protein structure of sequence from Norovirus. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for the recognition of blood group trisaccharides by norovirus., Cao S, Lou Z, Tan M, Chen Y, Liu Y, Zhang Z, Zhang XC, Jiang X, Li X, Rao Z, J Virol. 2007 Jun;81(11):5949-57. Epub 2007 Mar 28. PMID:17392366 Page seeded by OCA on Sun May 4 10:34:18 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2obt.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2obt |SIZE=350|CAPTION= <scene name='initialview01'>2obt</scene>, resolution 2.0&Aring;
+The line below this paragraph, containing "STRUCTURE_2obt", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GLB:BETA-D-GALACTOSE'>GLB</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2obt|  PDB=2obt  |  SCENE=  }}
-|RELATEDENTRY=[[2obr|2OBR]], [[2obs|2OBS]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2obt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2obt OCA], [http://www.ebi.ac.uk/pdbsum/2obt PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2obt RCSB]</span>
-}}
 '''Crystal Structures of P Domain of Norovirus VA387 in Complex with Blood Group Trisaccharides type B'''
@@ Line 28: / Line 25: @@
 [[Category: Li, X.]]
 [[Category: Rao, Z.]]
-[[Category: blood group trisaccharides type b]]
+[[Category: Blood group trisaccharides type b]]
-[[Category: crystal structure]]
+[[Category: Crystal structure]]
-[[Category: norovirus va387]]
+[[Category: Norovirus va387]]
-[[Category: p domain]]
+[[Category: P domain]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 10:34:18 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:15:31 2008''