2nyf: Difference between revisions

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Crystal structure of phenylalanine ammonia-lyase from Nostoc punctiforme

OverviewOverview

Phenylalanine ammonia lyase (PAL) catalyzes the deamination of phenylalanine to cinnamate and ammonia. While PALs are common in terrestrial plants where they catalyze the first committed step in the formation of phenylpropanoids, only a few prokaryotic PALs have been identified to date. Here we describe for the first time PALs from cyanobacteria, in particular, Anabaena variabilis ATCC 29413 and Nostoc punctiforme ATCC 29133, identified by screening the genome sequences of these organisms for members of the aromatic amino acid ammonia lyase family. Both PAL genes associate with secondary metabolite biosynthetic gene clusters as observed for other eubacterial PAL genes. In comparison to eukaryotic homologues, the cyanobacterial PALs are 20% smaller in size but share similar substrate selectivity and kinetic activity toward L-phenylalanine over L-tyrosine. Structure elucidation by protein X-ray crystallography confirmed that the two cyanobacterial PALs are similar in tertiary and quatenary structure to plant and yeast PALs as well as the mechanistically related histidine ammonia lyases.

About this StructureAbout this Structure

Full crystallographic information is available from OCA.

ReferenceReference

Discovery of two cyanobacterial phenylalanine ammonia lyases: kinetic and structural characterization., Moffitt MC, Louie GV, Bowman ME, Pence J, Noel JP, Moore BS, Biochemistry. 2007 Jan 30;46(4):1004-12. PMID:17240984 Page seeded by OCA on Sun May 4 10:04:35 2008

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OCA

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 [[Image:2nyf.gif|left|200px]]
- {{Structure
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phenylalanine_ammonia-lyase Phenylalanine ammonia-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.1.5 4.3.1.5] </span>
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-}}
 '''Crystal structure of phenylalanine ammonia-lyase from Nostoc punctiforme'''
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 ==About this Structure==
-NYF is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Nostoc_punctiforme Nostoc punctiforme]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NYF OCA].
+Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NYF OCA].
 ==Reference==
 Discovery of two cyanobacterial phenylalanine ammonia lyases: kinetic and structural characterization., Moffitt MC, Louie GV, Bowman ME, Pence J, Noel JP, Moore BS, Biochemistry. 2007 Jan 30;46(4):1004-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17240984 17240984]
-[[Category: Nostoc punctiforme]]
 [[Category: Phenylalanine ammonia-lyase]]
-[[Category: Protein complex]]
 [[Category: Bowman, M E.]]
 [[Category: Louie, G V.]]
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 [[Category: Noel, J P.]]
 [[Category: Pence, J.]]
-[[Category: methylidene imidazolone prosthetic group (autocatalytically formed by internal tripeptide segment ala167-ser168-gly169)]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 10:04:35 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:10:12 2008''