2nx7: Difference between revisions

Revision as of 10:01, 4 May 2008

Structure of NOWA cysteine rich domain 8

OverviewOverview

The nematocyst wall of cnidarians is a unique biomaterial that withstands extreme osmotic pressures, allowing an ultrafast discharge of the nematocyst capsules. Assembly of the highly robust nematocyst wall is achieved by covalent linkage of cysteine-rich domains (CRDs) from two main protein components, minicollagens and nematocyst outer wall antigen (NOWA). The bipolar minicollagens have different disulfide patterns and topologies in their N and C-terminal CRDs. The functional significance of this polarity has been elusive. Here, we show by NMR structural analysis that all representative cysteine-rich domains of NOWA are structurally related to N-terminal minicollagen domains. Natural sequence insertions in NOWA CRDs have very little effect on the tightly knit domain structures, nor do they preclude the efficient folding to a single native conformation. The different folds in NOWA CRDs and the atypical C-terminal minicollagen domain on the other hand can be directly related to different conformational preferences in the reduced states. Ultrastructural analysis in conjunction with aggregation studies argues for an association between the similar NOWA and N-terminal minicollagen domains in early stages of the nematocyst wall assembly, which is followed by the controlled association between the unusual structures of C-terminal minicollagen domains.

About this StructureAbout this Structure

2NX7 is a Single protein structure of sequence from Hydra vulgaris. Full crystallographic information is available from OCA.

ReferenceReference

Sequence-structure and structure-function analysis in cysteine-rich domains forming the ultrastable nematocyst wall., Meier S, Jensen PR, Adamczyk P, Bachinger HP, Holstein TW, Engel J, Ozbek S, Grzesiek S, J Mol Biol. 2007 May 4;368(3):718-28. Epub 2007 Feb 24. PMID:17362991 Page seeded by OCA on Sun May 4 10:01:39 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2nx7.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2nx7 |SIZE=350|CAPTION= <scene name='initialview01'>2nx7</scene>
+The line below this paragraph, containing "STRUCTURE_2nx7", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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+or leave the SCENE parameter empty for the default display.
-|GENE= NOWA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6087 Hydra vulgaris])
+-->
-|DOMAIN=
+{{STRUCTURE_2nx7|  PDB=2nx7  |  SCENE=  }}
-|RELATEDENTRY=[[2hm3|2HM3]], [[2nx6|2NX6]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nx7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nx7 OCA], [http://www.ebi.ac.uk/pdbsum/2nx7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2nx7 RCSB]</span>
-}}
 '''Structure of NOWA cysteine rich domain 8'''
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 [[Category: Meier, S.]]
 [[Category: Ozbek, S.]]
-[[Category: cysteine rich]]
+[[Category: Cysteine rich]]
-[[Category: disulfide bond]]
+[[Category: Disulfide bond]]
-[[Category: rich in turn]]
+[[Category: Rich in turn]]
-[[Category: structural protein]]
+[[Category: Structural protein]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 10:01:39 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:09:40 2008''