2nw9: Difference between revisions

Revision as of 09:59, 4 May 2008

Crystal Structure of Tryptophan 2,3-dioxygenase (TDO) from Xanthomonas campestris in complex with ferrous heme and 6-fluoro-tryptophan. Northeast Structural Genomics Target XcR13

OverviewOverview

Tryptophan 2,3-dioxygenase (TDO) and indoleamine 2,3-dioxygenase (IDO) constitute an important, yet relatively poorly understood, family of heme-containing enzymes. Here, we report extensive structural and biochemical studies of the Xanthomonas campestris TDO and a related protein SO4414 from Shewanella oneidensis, including the structure at 1.6-A resolution of the catalytically active, ferrous form of TDO in a binary complex with the substrate L-Trp. The carboxylate and ammonium moieties of tryptophan are recognized by electrostatic and hydrogen-bonding interactions with the enzyme and a propionate group of the heme, thus defining the L-stereospecificity. A second, possibly allosteric, L-Trp-binding site is present at the tetramer interface. The sixth coordination site of the heme-iron is vacant, providing a dioxygen-binding site that would also involve interactions with the ammonium moiety of L-Trp and the amide nitrogen of a glycine residue. The indole ring is positioned correctly for oxygenation at the C2 and C3 atoms. The active site is fully formed only in the binary complex, and biochemical experiments confirm this induced-fit behavior of the enzyme. The active site is completely devoid of water during catalysis, which is supported by our electrochemical studies showing significant stabilization of the enzyme upon substrate binding.

About this StructureAbout this Structure

2NW9 is a Single protein structure of sequence from Xanthomonas campestris pv. campestris. Full crystallographic information is available from OCA.

ReferenceReference

Molecular insights into substrate recognition and catalysis by tryptophan 2,3-dioxygenase., Forouhar F, Anderson JL, Mowat CG, Vorobiev SM, Hussain A, Abashidze M, Bruckmann C, Thackray SJ, Seetharaman J, Tucker T, Xiao R, Ma LC, Zhao L, Acton TB, Montelione GT, Chapman SK, Tong L, Proc Natl Acad Sci U S A. 2007 Jan 9;104(2):473-8. Epub 2006 Dec 29. PMID:17197414 Page seeded by OCA on Sun May 4 09:59:08 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2nw9.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_2nw9", creates the "Structure Box" on the page.
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=FT6:6-FLUORO-L-TRYPTOPHAN'>FT6</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>
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-|GENE= XCC0432 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=340 Xanthomonas campestris pv. campestris])
+-->
-|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG3483 TDO2]</span>
+{{STRUCTURE_2nw9|  PDB=2nw9  |  SCENE=  }}
-|RELATEDENTRY=[[1yw0|1YW0]], [[2nw7|2NW7]], [[2nw8|2NW8]], [[1zee|1ZEE]], [[2nwb|2NWB]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nw9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nw9 OCA], [http://www.ebi.ac.uk/pdbsum/2nw9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2nw9 RCSB]</span>
-}}
 '''Crystal Structure of Tryptophan 2,3-dioxygenase (TDO) from Xanthomonas campestris in complex with ferrous heme and 6-fluoro-tryptophan. Northeast Structural Genomics Target XcR13'''
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 [[Category: Xiao, R.]]
 [[Category: Zhao, L.]]
-[[Category: all alpha-helical protein]]
+[[Category: All alpha-helical protein]]
-[[Category: nesg]]
+[[Category: Nesg]]
-[[Category: northeast structural genomics consortium]]
+[[Category: Northeast structural genomics consortium]]
-[[Category: protein structure initiative]]
+[[Category: Protein structure initiative]]
-[[Category: psi-2]]
+[[Category: Psi-2]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 09:59:08 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:09:14 2008''