2nub: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2nub.jpg|left|200px]] | [[Image:2nub.jpg|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_2nub", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
or leave the SCENE parameter empty for the default display. | |||
--> | |||
{{STRUCTURE_2nub| PDB=2nub | SCENE= }} | |||
| | |||
}} | |||
'''Structure of Aquifex aeolicus Argonuate''' | '''Structure of Aquifex aeolicus Argonuate''' | ||
| Line 31: | Line 28: | ||
[[Category: Piehler, J.]] | [[Category: Piehler, J.]] | ||
[[Category: Rashid, U J.]] | [[Category: Rashid, U J.]] | ||
[[Category: | [[Category: Argonaute]] | ||
[[Category: | [[Category: Ribonuclease]] | ||
[[Category: | [[Category: Risc]] | ||
[[Category: | [[Category: Rnai]] | ||
[[Category: | [[Category: Rnaseh]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:55:13 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 09:55, 4 May 2008
Structure of Aquifex aeolicus Argonuate
OverviewOverview
Gene silencing mediated by RNA interference requires the sequence-specific recognition of target mRNA by the endonuclease Argonaute, the primary enzymatic component of the RNA-induced silencing complex. We report the crystal structure of Aquifex aeolicus Argonaute, refined at 3.2A resolution. Relative to recent Argonaute structures, a 24 degrees reorientation of the PAZ domain in our structure opens a basic cleft between the N-terminal and PAZ domains, exposing the guide strand binding pocket of PAZ. This rearrangement leads to a branched, Y-shaped system of grooves that extends through the molecule and merges in a central channel containing the catalytic residues. A 5.5-ns molecular dynamics simulation of Argonaute shows a strong tendency of the PAZ and N-terminal domains to be mobile. Binding of single-stranded DNA to Argonaute monitored by total internal reflection fluorescence spectroscopy shows biphasic kinetics, also indicative of domain rearrangement upon DNA binding. Conformational rearrangement of the PAZ domain may therefore be critical for the catalytic cycle of Argonaute and the RNA-induced silencing complex.
About this StructureAbout this Structure
2NUB is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.
ReferenceReference
Structure of Aquifex aeolicus argonaute highlights conformational flexibility of the PAZ domain as a potential regulator of RNA-induced silencing complex function., Rashid UJ, Paterok D, Koglin A, Gohlke H, Piehler J, Chen JC, J Biol Chem. 2007 May 4;282(18):13824-32. Epub 2006 Nov 27. PMID:17130125 Page seeded by OCA on Sun May 4 09:55:13 2008