2nmv: Difference between revisions

Revision as of 09:38, 4 May 2008

Damage detection by the UvrABC pathway: Crystal structure of UvrB bound to fluorescein-adducted DNA

OverviewOverview

UvrB is the damage recognition element of the highly conserved UvrABC pathway that functions in the removal of bulky DNA adducts. Pivotal to this is the formation of a damage detection complex that relies on the ability of UvrB to locate and sequester diverse lesions. Whilst structures of UvrB bound to DNA have recently been reported, none address the issue of lesion recognition. Here, we describe the crystal structure of UvrB bound to a pentanucleotide containing a single fluorescein-adducted thymine that reveals a unique mechanism for damage detection entirely dependent on the exclusion of lesions larger than an undamaged nucleotide.

About this StructureAbout this Structure

2NMV is a Protein complex structure of sequences from Bacillus subtilis. Full crystallographic information is available from OCA.

ReferenceReference

Damage detection by the UvrABC pathway: crystal structure of UvrB bound to fluorescein-adducted DNA., Waters TR, Eryilmaz J, Geddes S, Barrett TE, FEBS Lett. 2006 Nov 27;580(27):6423-7. Epub 2006 Nov 3. PMID:17097086 Page seeded by OCA on Sun May 4 09:38:44 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2nmv.gif|left|200px]]
- {{Structure
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-|GENE= uvrB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
+-->
-|DOMAIN=
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-|RELATEDENTRY=[[2d7d|2D7D]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nmv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nmv OCA], [http://www.ebi.ac.uk/pdbsum/2nmv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2nmv RCSB]</span>
-}}
 '''Damage detection by the UvrABC pathway: Crystal structure of UvrB bound to fluorescein-adducted DNA'''
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 [[Category: Geddes, S.]]
 [[Category: Waters, T R.]]
-[[Category: hairpin]]
+[[Category: Hairpin]]
-[[Category: protein-dna complex]]
+[[Category: Protein-dna complex]]
-[[Category: t-fluorescein]]
+[[Category: T-fluorescein]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 09:38:44 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:05:18 2008''