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'''DNUMB PTB DOMAIN COMPLEXED WITH A PHOSPHOTYROSINE PEPTIDE, NMR, ENSEMBLE OF STRUCTURES.''' | '''DNUMB PTB DOMAIN COMPLEXED WITH A PHOSPHOTYROSINE PEPTIDE, NMR, ENSEMBLE OF STRUCTURES.''' | ||
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[[Category: Vincent, S J.F.]] | [[Category: Vincent, S J.F.]] | ||
[[Category: Zwahlen, C.]] | [[Category: Zwahlen, C.]] | ||
[[Category: | [[Category: Asymetr ic cell division]] | ||
[[Category: | [[Category: Complex]] | ||
[[Category: | [[Category: Signal transduction]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:38:04 2008'' | |||
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Revision as of 09:38, 4 May 2008
DNUMB PTB DOMAIN COMPLEXED WITH A PHOSPHOTYROSINE PEPTIDE, NMR, ENSEMBLE OF STRUCTURES.
OverviewOverview
The phosphotyrosine-binding (PTB) domain of Numb, a protein involved in asymmetric cell division, has recently been shown to bind to the adapter protein Lnx through an LDNPAY sequence, to the Numb-associated kinase (Nak) through a sequence that does not contain an NPXY motif and to GP(p)Y-containing peptides obtained from library screening. We show here that these diverse peptide sequences bind with comparable affinities to the Numb PTB domain at a common binding site on the surface of the protein. The NMR structure of the Numb PTB domain in complex with a GPpY-containing peptide reveals a novel mechanism of binding with the peptide in a helical turn that does not hydrogen bond to the PTB domain beta-sheet. These results suggest that PTB domains can potentially have multiple modes of peptide recognition and provide a structural basis from which the multiple functions of the Numb PTB domain during asymmetric cell division could arise.
About this StructureAbout this Structure
2NMB is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
ReferenceReference
Structure of a Numb PTB domain-peptide complex suggests a basis for diverse binding specificity., Li SC, Zwahlen C, Vincent SJ, McGlade CJ, Kay LE, Pawson T, Forman-Kay JD, Nat Struct Biol. 1998 Dec;5(12):1075-83. PMID:9846878 Page seeded by OCA on Sun May 4 09:38:04 2008