2lyn: Difference between revisions

Revision as of 09:31, 4 May 2008

HIGH RESOLUTION STRUCTURE OF RED ABALONE LYSIN DIMER

OverviewOverview

Abalone sperm use lysin to make a hole in the egg's protective vitelline envelope (VE). When released from sperm, lysin first binds to the VE receptor for lysin (VERL) then dissolves the VE by a non-enzymatic mechanism. The structures of the monomeric and dimeric forms of Haliotis rufescens (red abalone) lysin, previously solved at 1.90 and 2.75 A, respectively, have now been refined to 1.35 and 2.07 A, respectively. The monomeric form of lysin was refined using previously obtained crystallization conditions, while the dimer was solved in a new crystal form with four molecules (two dimers) per asymmetric unit. These high-resolution structures reveal alternate residue conformations, enabling a thorough analysis of the conserved residues contributing to the amphipathic nature of lysin. The availability of five independent high-resolution copies of lysin permits comparisons leading to insights on the local flexibility of lysin and alternative conformations of the hypervariable N-terminus, thought to be involved in species-specific receptor recognition. The new analysis led to the discovery of the basic nature of a cleft formed upon dimerization and a patch of basic residues in the dimer interface. Identification of these features was not possible at lower resolution. In light of this new information, a model explaining the binding of sperm lysin to egg VERL and the subsequent dissolution of the egg VE is proposed.

About this StructureAbout this Structure

2LYN is a Single protein structure of sequence from Haliotis rufescens. Full crystallographic information is available from OCA.

ReferenceReference

1.35 and 2.07 A resolution structures of the red abalone sperm lysin monomer and dimer reveal features involved in receptor binding., Kresge N, Vacquier VD, Stout CD, Acta Crystallogr D Biol Crystallogr. 2000 Jan;56(Pt 1):34-41. PMID:10666624 Page seeded by OCA on Sun May 4 09:31:18 2008

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OCA

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 [[Image:2lyn.gif|left|200px]]
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 '''HIGH RESOLUTION STRUCTURE OF RED ABALONE LYSIN DIMER'''
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 [[Category: Stout, C D.]]
 [[Category: Vacquier, V D.]]
-[[Category: abalone lysin]]
+[[Category: Abalone lysin]]
-[[Category: fertilization protein]]
+[[Category: Fertilization protein]]
-[[Category: gamete recognition protein]]
+[[Category: Gamete recognition protein]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 09:31:18 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:03:38 2008''