2jv3: Difference between revisions
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'''Ets-1 PNT domain (29-138) NMR structure ensemble''' | '''Ets-1 PNT domain (29-138) NMR structure ensemble''' | ||
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==About this Structure== | ==About this Structure== | ||
2JV3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. This structure supersedes the now removed PDB entry | 2JV3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1bqv 1bqv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JV3 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Schaerpf, M.]] | [[Category: Schaerpf, M.]] | ||
[[Category: Slupsky, C M.]] | [[Category: Slupsky, C M.]] | ||
[[Category: | [[Category: Alpha-helical bundle]] | ||
[[Category: | [[Category: Dna-binding]] | ||
[[Category: | [[Category: Map kinase phosphorylation site]] | ||
[[Category: Nucleus]] | |||
[[Category: | [[Category: Transcription factor]] | ||
[[Category: | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:19:26 2008'' | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 09:19, 4 May 2008
Ets-1 PNT domain (29-138) NMR structure ensemble
OverviewOverview
The Pointed (PNT) domain and an adjacent mitogen-activated protein (MAP) kinase phosphorylation site are defined by sequence conservation among a subset of ets transcription factors and are implicated in two regulatory strategies, protein interactions and posttranslational modifications, respectively. By using NMR, we have determined the structure of a 110-residue fragment of murine Ets-1 that includes the PNT domain and MAP kinase site. The Ets-1 PNT domain forms a monomeric five-helix bundle. The architecture is distinct from that of any known DNA- or protein-binding module, including the helix-loop-helix fold proposed for the PNT domain of the ets protein TEL. The MAP kinase site is in a highly flexible region of both the unphosphorylated and phosphorylated forms of the Ets-1 fragment. Phosphorylation alters neither the structure nor monomeric state of the PNT domain. These results suggest that the Ets-1 PNT domain functions in heterotypic protein interactions and support the possibility that target recognition is coupled to structuring of the MAP kinase site.
About this StructureAbout this Structure
2JV3 is a Single protein structure of sequence from Mus musculus. This structure supersedes the now removed PDB entry 1bqv. Full crystallographic information is available from OCA.
ReferenceReference
Structure of the Ets-1 pointed domain and mitogen-activated protein kinase phosphorylation site., Slupsky CM, Gentile LN, Donaldson LW, Mackereth CD, Seidel JJ, Graves BJ, McIntosh LP, Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12129-34. PMID:9770451 Page seeded by OCA on Sun May 4 09:19:26 2008