2jnf: Difference between revisions

Revision as of 09:04, 4 May 2008

Solution structure of fly troponin C, isoform F1

OverviewOverview

To gain a molecular description of how muscles can be activated by mechanical stretch, we have solved the structure of the calcium-loaded F1 isoform of troponin C (TnC) from Lethocerus and characterized its interactions with troponin I (TnI). We show that the presence of only one calcium cation in the fourth EF hand motif is sufficient to induce an open conformation in the C-terminal lobe of F1 TnC, in contrast with what is observed in vertebrate muscle. This lobe interacts in a calcium-independent way both with the N terminus of TnI and, with lower affinity, with a region of TnI equivalent to the switch and inhibitory peptides of vertebrate muscles. Using both synthetic peptides and recombinant proteins, we show that the N lobe of F1 TnC is not engaged in interactions with TnI, excluding a regulatory role of this domain. These findings provide insights into mechanically stimulated muscle contraction.

About this StructureAbout this Structure

2JNF is a Single protein structure of sequence from Thermomicrobium roseum. Full crystallographic information is available from OCA.

ReferenceReference

The structure of Lethocerus troponin C: insights into the mechanism of stretch activation in muscles., De Nicola G, Burkart C, Qiu F, Agianian B, Labeit S, Martin S, Bullard B, Pastore A, Structure. 2007 Jul;15(7):813-24. PMID:17637342 Page seeded by OCA on Sun May 4 09:04:18 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2jnf.jpg|left|200px]]
- {{Structure
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-|GENE= tnC4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=500 Thermomicrobium roseum])
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-|DOMAIN=
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jnf OCA], [http://www.ebi.ac.uk/pdbsum/2jnf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2jnf RCSB]</span>
-}}
 '''Solution structure of fly troponin C, isoform F1'''
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 [[Category: Nicola, G F.De.]]
 [[Category: Pastore, A.]]
-[[Category: ef-hand]]
+[[Category: Ef-hand]]
-[[Category: lethocerus indicus]]
+[[Category: Lethocerus indicus]]
-[[Category: metal binding protein]]
+[[Category: Metal binding protein]]
-[[Category: stretch activated muscle contraction]]
+[[Category: Stretch activated muscle contraction]]
-[[Category: troponin c]]
+[[Category: Troponin c]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 09:04:18 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:59:41 2008''