2jaa: Difference between revisions

Revision as of 08:35, 4 May 2008

SEMET SUBSTITUTED SHIGELLA FLEXNERI IPAD

OverviewOverview

Bacteria expressing type III secretion systems (T3SS) have been responsible for the deaths of millions worldwide, acting as key virulence elements in diseases ranging from plague to typhoid fever. The T3SS is composed of a basal body, which traverses both bacterial membranes, and an external needle through which effector proteins are secreted. We report multiple crystal structures of two proteins that sit at the tip of the needle and are essential for virulence: IpaD from Shigella flexneri and BipD from Burkholderia pseudomallei. The structures reveal that the N-terminal domains of the molecules are intramolecular chaperones that prevent premature oligomerization, as well as sharing structural homology with proteins involved in eukaryotic actin rearrangement. Crystal packing has allowed us to construct a model for the tip complex that is supported by mutations designed using the structure.

About this StructureAbout this Structure

2JAA is a Single protein structure of sequence from Shigella flexneri. Full crystallographic information is available from OCA.

ReferenceReference

Self-chaperoning of the type III secretion system needle tip proteins IpaD and BipD., Johnson S, Roversi P, Espina M, Olive A, Deane JE, Birket S, Field T, Picking WD, Blocker AJ, Galyov EE, Picking WL, Lea SM, J Biol Chem. 2007 Feb 9;282(6):4035-44. Epub 2006 Oct 31. PMID:17077085 Page seeded by OCA on Sun May 4 08:35:50 2008

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OCA

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 [[Image:2jaa.jpg|left|200px]]
- {{Structure
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jaa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jaa OCA], [http://www.ebi.ac.uk/pdbsum/2jaa PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2jaa RCSB]</span>
-}}
 '''SEMET SUBSTITUTED SHIGELLA FLEXNERI IPAD'''
@@ Line 37: / Line 34: @@
 [[Category: Picking, W L.]]
 [[Category: Roversi, P.]]
-[[Category: cell invasion]]
+[[Category: Cell invasion]]
-[[Category: invasin]]
+[[Category: Invasin]]
-[[Category: ipad]]
+[[Category: Ipad]]
-[[Category: plasmid]]
+[[Category: Plasmid]]
-[[Category: semet]]
+[[Category: Semet]]
-[[Category: shigella flexneri]]
+[[Category: Shigella flexneri]]
-[[Category: t3ss]]
+[[Category: T3ss]]
-[[Category: type iii secretion]]
+[[Category: Type iii secretion]]
-[[Category: virulence]]
+[[Category: Virulence]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 08:35:50 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:55:19 2008''