2j55: Difference between revisions

Revision as of 08:20, 4 May 2008

X-RAY REDUCED PARACCOCUS DENITRIFICANS METHYLAMINE DEHYDROGENASE O-QUINONE IN COMPLEX WITH AMICYANIN.

OverviewOverview

X-ray exposure during crystallographic data collection can result in unintended redox changes in proteins containing functionally important redox centers. In order to directly monitor X-ray-derived redox changes in trapped oxidative half-reaction intermediates of Paracoccus denitrificans methylamine dehydrogenase, a commercially available single-crystal UV/Vis microspectrophotometer was installed on-line at the BioCARS beamline 14-BM-C at the Advanced Photon Source, Argonne, USA. Monitoring the redox state of the intermediates during X-ray exposure permitted the creation of a general multi-crystal data collection strategy to generate true structures of each redox intermediate.

About this StructureAbout this Structure

2J55 is a Protein complex structure of sequences from Paracoccus denitrificans. Full crystallographic information is available from OCA.

ReferenceReference

Tracking X-ray-derived redox changes in crystals of a methylamine dehydrogenase/amicyanin complex using single-crystal UV/Vis microspectrophotometry., Pearson AR, Pahl R, Kovaleva EG, Davidson VL, Wilmot CM, J Synchrotron Radiat. 2007 Jan;14(Pt 1):92-8. Epub 2006 Dec 15. PMID:17211075 Page seeded by OCA on Sun May 4 08:20:57 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2j55.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2j55 |SIZE=350|CAPTION= <scene name='initialview01'>2j55</scene>, resolution 2.15&Aring;
+The line below this paragraph, containing "STRUCTURE_2j55", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+L'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TRQ:2-AMINO-3-(6,7-DIOXO-6,7-DIHYDRO-1H-INDOL-3-YL)-PROPIONIC+ACID'>TRQ</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Amine_dehydrogenase Amine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.99.3 1.4.99.3] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2j55|  PDB=2j55  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j55 OCA], [http://www.ebi.ac.uk/pdbsum/2j55 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2j55 RCSB]</span>
-}}
 '''X-RAY REDUCED PARACCOCUS DENITRIFICANS METHYLAMINE DEHYDROGENASE O-QUINONE IN COMPLEX WITH AMICYANIN.'''
@@ Line 30: / Line 27: @@
 [[Category: Pearson, A R.]]
 [[Category: Wilmot, C M.]]
-[[Category: copper]]
+[[Category: Copper]]
-[[Category: electron transport]]
+[[Category: Electron transport]]
-[[Category: metal-binding]]
+[[Category: Metal-binding]]
-[[Category: methylamine dehydrogenase single crystal microspectrophoto]]
+[[Category: Methylamine dehydrogenase single crystal microspectrophoto]]
-[[Category: oxidoreductase]]
+[[Category: Oxidoreductase]]
-[[Category: periplasmic]]
+[[Category: Periplasmic]]
-[[Category: transport]]
+[[Category: Transport]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 08:20:57 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:53:03 2008''