2j4d: Difference between revisions

Revision as of 08:18, 4 May 2008

CRYPTOCHROME 3 FROM ARABIDOPSIS THALIANA

OverviewOverview

Cryptochromes are almost ubiquitous blue-light receptors and act in several species as central components of the circadian clock. Despite being evolutionary and structurally related with DNA photolyases, a class of light-driven DNA-repair enzymes, and having similar cofactor compositions, cryptochromes lack DNA-repair activity. Cryptochrome 3 from the plant Arabidopsis thaliana belongs to the DASH-type subfamily. Its crystal structure determined at 1.9 Angstroms resolution shows cryptochrome 3 in a dimeric state with the antenna cofactor 5,10-methenyltetrahydrofolate (MTHF) bound in a distance of 15.2 Angstroms to the U-shaped FAD chromophore. Spectroscopic studies on a mutant where a residue crucial for MTHF-binding, E149, was replaced by site-directed mutagenesis demonstrate that MTHF acts in cryptochrome 3 as a functional antenna for the photoreduction of FAD.

About this StructureAbout this Structure

2J4D is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.

ReferenceReference

Cryptochrome 3 from Arabidopsis thaliana: structural and functional analysis of its complex with a folate light antenna., Klar T, Pokorny R, Moldt J, Batschauer A, Essen LO, J Mol Biol. 2007 Feb 23;366(3):954-64. Epub 2006 Dec 2. PMID:17188299 Page seeded by OCA on Sun May 4 08:18:51 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2j4d.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2j4d |SIZE=350|CAPTION= <scene name='initialview01'>2j4d</scene>, resolution 1.9&Aring;
+The line below this paragraph, containing "STRUCTURE_2j4d", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Mhf+Binding+Site+For+Chain+B'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MHF:5,10-METHENYL-6,7,8-TRIHYDROFOLIC+ACID'>MHF</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2j4d|  PDB=2j4d  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j4d OCA], [http://www.ebi.ac.uk/pdbsum/2j4d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2j4d RCSB]</span>
-}}
 '''CRYPTOCHROME 3 FROM ARABIDOPSIS THALIANA'''
@@ Line 29: / Line 26: @@
 [[Category: Klar, T.]]
 [[Category: Pokorny, R.]]
-[[Category: blue-light response]]
+[[Category: Blue-light response]]
-[[Category: chloroplast]]
+[[Category: Chloroplast]]
-[[Category: chromophore]]
+[[Category: Chromophore]]
-[[Category: cryptochrome]]
+[[Category: Cryptochrome]]
-[[Category: dna-binding protein]]
+[[Category: Dna-binding protein]]
-[[Category: fad]]
+[[Category: Fad]]
-[[Category: flavoprotein]]
+[[Category: Flavoprotein]]
-[[Category: mitochondrion]]
+[[Category: Mitochondrion]]
-[[Category: plastid]]
+[[Category: Plastid]]
-[[Category: transit peptide]]
+[[Category: Transit peptide]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 08:18:51 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:52:44 2008''