2izx: Difference between revisions

Revision as of 08:08, 4 May 2008

MOLECULAR BASIS OF AKAP SPECIFICITY FOR PKA REGULATORY SUBUNITS

OverviewOverview

Localization of cyclic AMP (cAMP)-dependent protein kinase (PKA) by A kinase-anchoring proteins (AKAPs) restricts the action of this broad specificity kinase. The high-resolution crystal structures of the docking and dimerization (D/D) domain of the RIIalpha regulatory subunit of PKA both in the apo state and in complex with the high-affinity anchoring peptide AKAP-IS explain the molecular basis for AKAP-regulatory subunit recognition. AKAP-IS folds into an amphipathic alpha helix that engages an essentially preformed shallow groove on the surface of the RII dimer D/D domains. Conserved AKAP aliphatic residues dominate interactions to RII at the predominantly hydrophobic interface, whereas polar residues are important in conferring R subunit isoform specificity. Using a peptide screening approach, we have developed SuperAKAP-IS, a peptide that is 10,000-fold more selective for the RII isoform relative to RI and can be used to assess the impact of PKA isoform-selective anchoring on cAMP-responsive events inside cells.

About this StructureAbout this Structure

2IZX is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Molecular basis of AKAP specificity for PKA regulatory subunits., Gold MG, Lygren B, Dokurno P, Hoshi N, McConnachie G, Tasken K, Carlson CR, Scott JD, Barford D, Mol Cell. 2006 Nov 3;24(3):383-95. PMID:17081989 Page seeded by OCA on Sun May 4 08:08:20 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2izx.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2izx |SIZE=350|CAPTION= <scene name='initialview01'>2izx</scene>, resolution 1.30&Aring;
+The line below this paragraph, containing "STRUCTURE_2izx", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Dtd+Binding+Site+For+Chain+A'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=DTD:DITHIANE+DIOL'>DTD</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/cAMP-dependent_protein_kinase cAMP-dependent protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.11 2.7.11.11] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2izx|  PDB=2izx  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2izx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2izx OCA], [http://www.ebi.ac.uk/pdbsum/2izx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2izx RCSB]</span>
-}}
 '''MOLECULAR BASIS OF AKAP SPECIFICITY FOR PKA REGULATORY SUBUNITS'''
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 [[Category: Homo sapiens]]
 [[Category: Single protein]]
-[[Category: cAMP-dependent protein kinase]]
+[[Category: CAMP-dependent protein kinase]]
 [[Category: Barford, D.]]
 [[Category: Carlson, C R.]]
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 [[Category: Scott, J D.]]
 [[Category: Tasken, K.]]
-[[Category: acetylation]]
+[[Category: Acetylation]]
-[[Category: akap]]
+[[Category: Akap]]
-[[Category: anchor]]
+[[Category: Anchor]]
-[[Category: camp]]
+[[Category: Camp]]
-[[Category: camp-binding]]
+[[Category: Camp-binding]]
-[[Category: kinase]]
+[[Category: Kinase]]
-[[Category: nucleotide-binding]]
+[[Category: Nucleotide-binding]]
-[[Category: phosphorylation]]
+[[Category: Phosphorylation]]
-[[Category: pka]]
+[[Category: Pka]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 08:08:20 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:51:05 2008''