2iyv: Difference between revisions

Revision as of 08:05, 4 May 2008

SHIKIMATE KINASE FROM MYCOBACTERIUM TUBERCULOSIS IN COMPLEX WITH ADP, OPEN LID (CONF. B)

OverviewOverview

The structural mechanism of the catalytic functioning of shikimate kinase from Mycobacterium tuberculosis was investigated on the basis of a series of high-resolution crystal structures corresponding to individual steps in the enzymatic reaction. The catalytic turnover of shikimate and ATP into the products shikimate-3-phosphate and ADP, followed by release of ADP, was studied in the crystalline environment. Based on a comparison of the structural states before initiation of the reaction and immediately after the catalytic step, we derived a structural model of the transition state that suggests that phosphoryl transfer proceeds with inversion by an in-line associative mechanism. The random sequential binding of shikimate and nucleotides is associated with domain movements. We identified a synergic mechanism by which binding of the first substrate may enhance the affinity for the second substrate.

About this StructureAbout this Structure

2IYV is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

ReferenceReference

Mechanism of phosphoryl transfer catalyzed by shikimate kinase from Mycobacterium tuberculosis., Hartmann MD, Bourenkov GP, Oberschall A, Strizhov N, Bartunik HD, J Mol Biol. 2006 Dec 1;364(3):411-23. Epub 2006 Sep 5. PMID:17020768 Page seeded by OCA on Sun May 4 08:05:51 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2iyv.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2iyv |SIZE=350|CAPTION= <scene name='initialview01'>2iyv</scene>, resolution 1.35&Aring;
+The line below this paragraph, containing "STRUCTURE_2iyv", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Cl+Binding+Site+For+Chain+A'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Shikimate_kinase Shikimate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.71 2.7.1.71] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2iyv|  PDB=2iyv  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iyv OCA], [http://www.ebi.ac.uk/pdbsum/2iyv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2iyv RCSB]</span>
-}}
 '''SHIKIMATE KINASE FROM MYCOBACTERIUM TUBERCULOSIS IN COMPLEX WITH ADP, OPEN LID (CONF. B)'''
@@ Line 31: / Line 28: @@
 [[Category: Oberschall, A.]]
 [[Category: Strizhov, N.]]
-[[Category: amino-acid biosynthesis]]
+[[Category: Amino-acid biosynthesis]]
-[[Category: aromatic amino acid biosynthesis]]
+[[Category: Aromatic amino acid biosynthesis]]
-[[Category: atp-binding]]
+[[Category: Atp-binding]]
-[[Category: kinase]]
+[[Category: Kinase]]
-[[Category: magnesium]]
+[[Category: Magnesium]]
-[[Category: metal-binding]]
+[[Category: Metal-binding]]
-[[Category: nucleotide-binding]]
+[[Category: Nucleotide-binding]]
-[[Category: p-loop kinase]]
+[[Category: P-loop kinase]]
-[[Category: shikimate kinase]]
+[[Category: Shikimate kinase]]
-[[Category: shikimate pathway]]
+[[Category: Shikimate pathway]]
-[[Category: transferase]]
+[[Category: Transferase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 08:05:51 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:50:40 2008''