2iu6: Difference between revisions

Revision as of 07:53, 4 May 2008

REGULATION OF THE DHA OPERON OF LACTOCOCCUS LACTIS

OverviewOverview

Dihydroxyacetone (Dha) kinases are a novel family of kinases with signaling and metabolic functions. Here we report the x-ray structures of the transcriptional activator DhaS and the coactivator DhaQ and characterize their function. DhaQ is a paralog of the Dha binding Dha kinase subunit; DhaS belongs to the family of TetR repressors although, unlike all known members of this family, it is a transcriptional activator. DhaQ and DhaS form a stable complex that in the presence of Dha activates transcription of the Lactococcus lactis dha operon. Dha covalently binds to DhaQ through a hemiaminal bond with a histidine and thereby induces a conformational change, which is propagated to the surface via a cantilever-like structure. DhaS binding protects an inverted repeat whose sequence is GGACACATN6ATTTGTCC and renders two GC base pairs of the operator DNA hypersensitive to DNase I cleavage. The proximal half-site of the inverted repeat partially overlaps with the predicted -35 consensus sequence of the dha promoter.

About this StructureAbout this Structure

2IU6 is a Single protein structure of sequence from Lactococcus lactis. Full crystallographic information is available from OCA.

ReferenceReference

Regulation of the Dha operon of Lactococcus lactis: a deviation from the rule followed by the Tetr family of transcription regulators., Christen S, Srinivas A, Bahler P, Zeller A, Pridmore D, Bieniossek C, Baumann U, Erni B, J Biol Chem. 2006 Aug 11;281(32):23129-37. Epub 2006 Jun 7. PMID:16760471 Page seeded by OCA on Sun May 4 07:53:06 2008

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OCA

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 [[Image:2iu6.gif|left|200px]]
- {{Structure
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span>
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iu6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iu6 OCA], [http://www.ebi.ac.uk/pdbsum/2iu6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2iu6 RCSB]</span>
-}}
 '''REGULATION OF THE DHA OPERON OF LACTOCOCCUS LACTIS'''
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 [[Category: Erni, B.]]
 [[Category: Srinivas, A.]]
-[[Category: dihydroxyacetone kinase transferase]]
+[[Category: Dihydroxyacetone kinase transferase]]
-[[Category: kinase]]
+[[Category: Kinase]]
-[[Category: transferase]]
+[[Category: Transferase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 07:53:06 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:48:38 2008''