2is6: Difference between revisions

Revision as of 07:48, 4 May 2008

Crystal structure of UvrD-DNA-ADPMgF3 ternary complex

OverviewOverview

Helicases use the energy derived from nucleoside triphosphate hydrolysis to unwind double helices in essentially every metabolic pathway involving nucleic acids. Earlier crystal structures have suggested that DNA helicases translocate along a single-stranded DNA in an inchworm fashion. We report here a series of crystal structures of the UvrD helicase complexed with DNA and ATP hydrolysis intermediates. These structures reveal that ATP binding alone leads to unwinding of 1 base pair by directional rotation and translation of the DNA duplex, and ADP and Pi release leads to translocation of the developing single strand. Thus DNA unwinding is achieved by a two-part power stroke in a combined wrench-and-inchworm mechanism. The rotational angle and translational distance of DNA define the unwinding step to be 1 base pair per ATP hydrolyzed. Finally, a gateway for ssDNA translocation and an alternative strand-displacement mode may explain the varying step sizes reported previously.

About this StructureAbout this Structure

2IS6 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

UvrD helicase unwinds DNA one base pair at a time by a two-part power stroke., Lee JY, Yang W, Cell. 2006 Dec 29;127(7):1349-60. PMID:17190599 Page seeded by OCA on Sun May 4 07:48:11 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2is6.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2is6 |SIZE=350|CAPTION= <scene name='initialview01'>2is6</scene>, resolution 2.20&Aring;
+The line below this paragraph, containing "STRUCTURE_2is6", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MGF:TRIFLUOROMAGNESATE'>MGF</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= uvrD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+-->
-|DOMAIN=
+{{STRUCTURE_2is6|  PDB=2is6  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2is6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2is6 OCA], [http://www.ebi.ac.uk/pdbsum/2is6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2is6 RCSB]</span>
-}}
 '''Crystal structure of UvrD-DNA-ADPMgF3 ternary complex'''
@@ Line 27: / Line 24: @@
 [[Category: Lee, J Y.]]
 [[Category: Yang, W.]]
-[[Category: dna helicase]]
+[[Category: Dna helicase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 07:48:11 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:47:48 2008''