2ifu: Difference between revisions

Revision as of 07:27, 4 May 2008

Crystal Structure of a Gamma-SNAP from Danio rerio

OverviewOverview

Soluble N-ethylmaleimide-sensitive factor attachment protein gamma (gamma-SNAP) is a member of an eukaryotic protein family involved in intracellular membrane trafficking. The X-ray structure of Brachydanio rerio gamma-SNAP was determined to 2.6 A and revealed an all-helical protein comprised of an extended twisted-sheet of helical hairpins with a helical-bundle domain on its carboxy-terminal end. Structural and conformational differences between multiple observed gamma-SNAP molecules and Sec17, a SNAP family protein from yeast, are analyzed. Conformational variation in gamma-SNAP molecules is matched with great precision by the two lowest frequency normal modes of the structure. Comparison of the lowest-frequency modes from gamma-SNAP and Sec17 indicated that the structures share preferred directions of flexibility, corresponding to bending and twisting of the twisted sheet motif. We discuss possible consequences related to the flexibility of the SNAP proteins for the mechanism of the 20S complex disassembly during the SNAP receptors recycling.

About this StructureAbout this Structure

2IFU is a Single protein structure of sequence from Danio rerio. Full crystallographic information is available from OCA.

ReferenceReference

Structure and dynamics of gamma-SNAP: insight into flexibility of proteins from the SNAP family., Bitto E, Bingman CA, Kondrashov DA, McCoy JG, Bannen RM, Wesenberg GE, Phillips GN Jr, Proteins. 2008 Jan 1;70(1):93-104. PMID:17634982 Page seeded by OCA on Sun May 4 07:27:15 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2ifu.gif|left|200px]]
- {{Structure
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-|GENE= zgc:110177 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7955 Danio rerio])
+-->
-|DOMAIN=
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ifu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ifu OCA], [http://www.ebi.ac.uk/pdbsum/2ifu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ifu RCSB]</span>
-}}
 '''Crystal Structure of a Gamma-SNAP from Danio rerio'''
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 [[Category: Mccoy, J G.]]
 [[Category: Wesenberg, G E.]]
-[[Category: center for eukaryotic structural genomic]]
+[[Category: Center for eukaryotic structural genomic]]
-[[Category: cesg]]
+[[Category: Cesg]]
-[[Category: endocytosis/exocytosis complex]]
+[[Category: Endocytosis/exocytosis complex]]
-[[Category: gamma-snap]]
+[[Category: Gamma-snap]]
-[[Category: membrane fusion]]
+[[Category: Membrane fusion]]
-[[Category: protein structure initiative]]
+[[Category: Protein structure initiative]]
-[[Category: psi]]
+[[Category: Psi]]
-[[Category: snare complex disassembly]]
+[[Category: Snare complex disassembly]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 07:27:15 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:43:44 2008''