2i3b: Difference between revisions

Revision as of 07:01, 4 May 2008

Solution Structure of a Human Cancer-Related Nucleoside Triphosphatase

OverviewOverview

A screen of the human cancer genome anatomy project (CGAP) database was performed to search for new proteins involved in tumorigenesis. The resulting hits were further screened for recombinant expression, solubility and protein aggregation, which led to the identification of the previously unknown human cancer-related (HCR) protein encoded by the mRNA NM_032324 as a target for structure determination by NMR. The three-dimensional structure of the protein in its complex with ATPgammaS forms a three-layered alpha/beta sandwich, with a central nine-stranded beta-sheet surrounded by five alpha-helices. Sequence and three-dimensional structure comparisons with AAA+ ATPases revealed the presence of Walker A (GPPGVGKT) and Walker B (VCVIDEIG) motifs. Using 1D (31)P-NMR spectroscopy and a coupled enzymatic assay for the determination of inorganic phosphate, we showed that the purified recombinant protein is active as a non-specific nucleoside triphosphatase, with k(cat)=7.6x10(-3) s(-1). The structural basis for the enzymatic activity of HCR-NTPase was further characterized by site-directed mutagenesis of the Walker B motif, which further contributes to making the HCR-NTPase an attractive new target for further biochemical characterization in the context of its presumed role in human tumorigenesis.

About this StructureAbout this Structure

2I3B is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

NMR structure and functional characterization of a human cancer-related nucleoside triphosphatase., Placzek WJ, Almeida MS, Wuthrich K, J Mol Biol. 2007 Mar 30;367(3):788-801. Epub 2007 Jan 9. PMID:17291528 Page seeded by OCA on Sun May 4 07:01:13 2008

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OCA

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 [[Image:2i3b.jpg|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_2i3b", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nucleoside-triphosphatase Nucleoside-triphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.15 3.6.1.15] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= CDRT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+-->
-|DOMAIN=
+{{STRUCTURE_2i3b|  PDB=2i3b  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i3b OCA], [http://www.ebi.ac.uk/pdbsum/2i3b PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2i3b RCSB]</span>
-}}
 '''Solution Structure of a Human Cancer-Related Nucleoside Triphosphatase'''
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 [[Category: Placzek, W J.]]
 [[Category: Wuthrich, K.]]
-[[Category: aaa]]
+[[Category: Aaa]]
-[[Category: ntpase]]
+[[Category: Ntpase]]
-[[Category: rossmann]]
+[[Category: Rossmann]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 07:01:13 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:39:09 2008''