2i2z: Difference between revisions

Revision as of 07:00, 4 May 2008

Human serum albumin complexed with myristate and aspirin

OverviewOverview

Human serum albumin (HSA) is the most abundant plasma protein in the human body with a plasma concentration of 0.6mM. HSA plays an important role in drug transport and metabolism. Enzymatic activity of HSA on different substrates or drugs has been studied and documented. The structural mechanism of this activity, however, is unknown. In this study, we have determined the crystal structures of HSA-myristate in a complex of aspirin and of salicylic acid, respectively. The crystal structure of HSA-myristate-aspirin illustrates that aspirin transfers acetyl group to Lys199 and is hydrolyzed into salicylic acid by HSA. The hydrolysis product, salicylic acid, remains bound to HSA at a similar location, but it shows a very different orientation when compared with the salicylic acid in the HSA-myristate-salicylic acid ternary complex. These results not only provide the structural evidence of esterase activity of HSA, and demonstrate the conformational plasticity of HSA on drug binding, but also may provide structural information for the modulation of HSA-drug interaction by computational approach based on HSA-drug structure.

About this StructureAbout this Structure

2I2Z is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Effect of human serum albumin on drug metabolism: structural evidence of esterase activity of human serum albumin., Yang F, Bian C, Zhu L, Zhao G, Huang Z, Huang M, J Struct Biol. 2007 Feb;157(2):348-55. Epub 2006 Sep 9. PMID:17067818 Page seeded by OCA on Sun May 4 07:00:35 2008

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OCA

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 [[Image:2i2z.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2i2z |SIZE=350|CAPTION= <scene name='initialview01'>2i2z</scene>, resolution 2.700&Aring;
+The line below this paragraph, containing "STRUCTURE_2i2z", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene>, <scene name='pdbligand=MYR:MYRISTIC+ACID'>MYR</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2i2z|  PDB=2i2z  |  SCENE=  }}
-|RELATEDENTRY=[[2bxl|2BXL]], [[2i30|2I30]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i2z OCA], [http://www.ebi.ac.uk/pdbsum/2i2z PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2i2z RCSB]</span>
-}}
 '''Human serum albumin complexed with myristate and aspirin'''
@@ Line 31: / Line 28: @@
 [[Category: Zhao, G.]]
 [[Category: Zhu, L.]]
-[[Category: lipid-binding]]
+[[Category: Lipid-binding]]
-[[Category: metal-binding]]
+[[Category: Metal-binding]]
-[[Category: plasma protein]]
+[[Category: Plasma protein]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 07:00:35 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:38:58 2008''