2hla: Difference between revisions

Revision as of 06:25, 4 May 2008

SPECIFICITY POCKETS FOR THE SIDE CHAINS OF PEPTIDE ANTIGENS IN HLA-AW68

OverviewOverview

We have determined the structure of a second human histocompatibility glycoprotein, HLA-Aw68, by X-ray crystallography and refined it to a resolution of 2.6 A. Overall, the structure is extremely similar to that of HLA-A2 (refs 1, 2; and M.A.S. et al., manuscript in preparation), although the 11 amino-acid substitutions at polymorphic residues in the antigen-binding cleft alter the detailed shape and electrostatic charge of that site. A prominent negatively charged pocket within the cleft extends underneath the alpha-helix of the alpha 1-domain, providing a potential subsite for recognizing a positively charged side chain or peptide N terminus. Uninterpreted electron density, presumably representing an unknown 'antigen(s)', which seems to be different from that seen in the HLA-A2 structure, occupies the cleft and extends into the negatively charged pocket in HLA-Aw68. The structures of HLA-Aw68 and HLA-A2 demonstrate how polymorphism creates and alters subsites (pockets) positioned to bind peptide side chains, thereby suggesting the structural basis for allelic specificity in foreign antigen binding.

About this StructureAbout this Structure

2HLA is a Protein complex structure of sequences from Homo sapiens. The following page contains interesting information on the relation of 2HLA with [Major Histocompatibility Complex]. Full crystallographic information is available from OCA.

ReferenceReference

Specificity pockets for the side chains of peptide antigens in HLA-Aw68., Garrett TP, Saper MA, Bjorkman PJ, Strominger JL, Wiley DC, Nature. 1989 Dec 7;342(6250):692-6. PMID:2594067 Page seeded by OCA on Sun May 4 06:25:26 2008

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OCA

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 [[Image:2hla.gif|left|200px]]
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 '''SPECIFICITY POCKETS FOR THE SIDE CHAINS OF PEPTIDE ANTIGENS IN HLA-AW68'''
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 [[Category: Saper, M A.]]
 [[Category: Wiley, D C.]]
-[[Category: histocompatibility antigen]]
+[[Category: Histocompatibility antigen]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 06:25:26 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:31:54 2008''