2hk8: Difference between revisions

Revision as of 06:23, 4 May 2008

Crystal structure of shikimate dehydrogenase from aquifex aeolicus at 2.35 angstrom resolution

OverviewOverview

The shikimate biosynthetic pathway is essential to microorganisms, plants, and parasites but absent from mammals. Therefore, shikimate dehydrogenase (SD) and other enzymes in the pathway are attractive targets for developing nontoxic antimicrobial agents, herbicides, and antiparasite drugs. SD catalyzes the fourth reaction in the pathway, the nicotinamide adenine dinucleotide phosphate- (NADP-) dependent reduction of 3-dehydroshikimic acid to shikimic acid (SA), as well as its reverse, by the transfer of a hydride. Previous structural studies reveal that the enzyme exists in two major conformations, an open and a closed form. For the reaction to occur, it is believed that the catalytic complex assumes the closed conformation. Nonetheless, the only structure containing both SA and NADP+ exhibits an open conformation (PDB entry 2EV9). Here, we present two crystal structures of Aquifex aeolicus SD, including a ternary complex with both SA and NADP+, which assumes the closed conformation and therefore contains a catalytically competent active site. On the basis of preexisting and novel structural and biochemical data, a catalytic mechanism is proposed.

About this StructureAbout this Structure

2HK8 is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.

ReferenceReference

Structural and biochemical analyses of shikimate dehydrogenase AroE from Aquifex aeolicus: implications for the catalytic mechanism., Gan J, Wu Y, Prabakaran P, Gu Y, Li Y, Andrykovitch M, Liu H, Gong Y, Yan H, Ji X, Biochemistry. 2007 Aug 21;46(33):9513-22. Epub 2007 Jul 25. PMID:17649975 Page seeded by OCA on Sun May 4 06:23:19 2008

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OCA

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 [[Image:2hk8.jpg|left|200px]]
- {{Structure
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-|PDB= 2hk8 |SIZE=350|CAPTION= <scene name='initialview01'>2hk8</scene>, resolution 2.35&Aring;
+The line below this paragraph, containing "STRUCTURE_2hk8", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Shikimate_dehydrogenase Shikimate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.25 1.1.1.25] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= aroE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus])
+-->
-|DOMAIN=
+{{STRUCTURE_2hk8|  PDB=2hk8  |  SCENE=  }}
-|RELATEDENTRY=[[2ev9|2EV9]], [[1wxd|1WXD]], [[2hk7|2HK7]], [[2hk9|2HK9]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hk8 OCA], [http://www.ebi.ac.uk/pdbsum/2hk8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2hk8 RCSB]</span>
-}}
 '''Crystal structure of shikimate dehydrogenase from aquifex aeolicus at 2.35 angstrom resolution'''
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 [[Category: Prabakaran, P.]]
 [[Category: Yan, H.]]
-[[Category: drug design]]
+[[Category: Drug design]]
-[[Category: oxidoreductase]]
+[[Category: Oxidoreductase]]
-[[Category: shikimate dehydrogenase]]
+[[Category: Shikimate dehydrogenase]]
-[[Category: shikimate pathway]]
+[[Category: Shikimate pathway]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 06:23:19 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:31:30 2008''