2hdc: Difference between revisions

Revision as of 06:08, 4 May 2008

STRUCTURE OF TRANSCRIPTION FACTOR GENESIS/DNA COMPLEX

OverviewOverview

Genesis is an HNF-3/fkh homologous protein. By using multi-dimensional NMR techniques, we have obtained the solution structure and backbone dynamics of Genesis complexed with a 17 base-pair DNA. Our results indicate that both the local folding and dynamic properties of Genesis are perturbed when it binds to the DNA site. Our data show that a conserved flexible amino acid sequence (wing 1) makes dynamic contacts to DNA in the complex and a short helix is induced by Genesis-DNA interactions. Our data indicate that, unlike the HNF-3gamma/DNA complex, a magnesium ion is not required in forming the stable Genesis-DNA complex.

About this StructureAbout this Structure

2HDC is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

ReferenceReference

Dynamic DNA contacts observed in the NMR structure of winged helix protein-DNA complex., Jin C, Marsden I, Chen X, Liao X, J Mol Biol. 1999 Jun 18;289(4):683-90. PMID:10369754 Page seeded by OCA on Sun May 4 06:08:55 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2hdc.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2hdc |SIZE=350|CAPTION= <scene name='initialview01'>2hdc</scene>
+The line below this paragraph, containing "STRUCTURE_2hdc", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= GENESIS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])
+-->
-|DOMAIN=
+{{STRUCTURE_2hdc|  PDB=2hdc  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hdc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hdc OCA], [http://www.ebi.ac.uk/pdbsum/2hdc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2hdc RCSB]</span>
-}}
 '''STRUCTURE OF TRANSCRIPTION FACTOR GENESIS/DNA COMPLEX'''
@@ Line 29: / Line 26: @@
 [[Category: Liao, X.]]
 [[Category: Marsden, I.]]
-[[Category: dyanamic]]
+[[Category: Dyanamic]]
-[[Category: genesis]]
+[[Category: Genesis]]
-[[Category: nmr]]
+[[Category: Nmr]]
-[[Category: structure]]
+[[Category: Structure]]
-[[Category: winged helix protein]]
+[[Category: Winged helix protein]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 06:08:55 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:28:45 2008''