2h9m: Difference between revisions

Revision as of 06:01, 4 May 2008

WDR5 in complex with unmodified H3K4 peptide

OverviewOverview

Histone methylation at specific lysine residues brings about various downstream events that are mediated by different effector proteins. The WD40 domain of WDR5 represents a new class of histone methyl-lysine recognition domains that is important for recruiting H3K4 methyltransferases to K4-dimethylated histone H3 tail as well as for global and gene-specific K4 trimethylation. Here we report the crystal structures of full-length WDR5, WDR5Delta23 and its complexes with unmodified, mono-, di- and trimethylated histone H3K4 peptides. The structures reveal that WDR5 is able to bind all of these histone H3 peptides, but only H3K4me2 peptide forms extra interactions with WDR5 by use of both water-mediated hydrogen bonding and the altered hydrophilicity of the modified lysine 4. We propose a mechanism for the involvement of WDR5 in binding and presenting histone H3K4 for further methylation as a component of MLL complexes.

About this StructureAbout this Structure

2H9M is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for molecular recognition and presentation of histone H3 by WDR5., Schuetz A, Allali-Hassani A, Martin F, Loppnau P, Vedadi M, Bochkarev A, Plotnikov AN, Arrowsmith CH, Min J, EMBO J. 2006 Sep 20;25(18):4245-52. Epub 2006 Aug 31. PMID:16946699 Page seeded by OCA on Sun May 4 06:01:33 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2h9m.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_2h9m", creates the "Structure Box" on the page.
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-|GENE= WDR5, BIG3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+-->
-|DOMAIN=
+{{STRUCTURE_2h9m|  PDB=2h9m  |  SCENE=  }}
-|RELATEDENTRY=[[2h9l|2H9L]], [[2h9n|2H9N]], [[2h9o|2H9O]], [[2h9p|2H9P]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h9m OCA], [http://www.ebi.ac.uk/pdbsum/2h9m PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2h9m RCSB]</span>
-}}
 '''WDR5 in complex with unmodified H3K4 peptide'''
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 [[Category: Vedadi, M.]]
 [[Category: Weigelt, J.]]
-[[Category: sgc]]
+[[Category: Sgc]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
-[[Category: structural genomics consortium]]
+[[Category: Structural genomics consortium]]
-[[Category: wdr5]]
+[[Category: Wdr5]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 06:01:33 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:27:20 2008''