2h8c: Difference between revisions

Revision as of 05:59, 4 May 2008

Structure of RusA D70N in complex with DNA

OverviewOverview

We have determined the structure of a catalytically inactive D70N variant of the Escherichia coli RusA resolvase bound to a duplex DNA substrate that reveals critical protein-DNA interactions and permits a much clearer understanding of the interaction of the enzyme with a Holliday junction (HJ). The RusA enzyme cleaves HJs, the fourway DNA branchpoints formed by homologous recombination, by introducing symmetrical cuts in the phosphodiester backbone in a Mg2+ dependent reaction. Although, RusA shows a high level of selectivity for DNA junctions, preferring to bind fourway junctions over other substrates in vitro, it has also been shown to have appreciable affinity for duplex DNA. However, RusA does not show DNA cleavage activity with duplex substrates. Our structure suggests the possible basis for structural selectivity as well as sources of the sequence specificity observed for DNA cleavage by RusA.

About this StructureAbout this Structure

2H8C is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

RusA Holliday junction resolvase: DNA complex structure--insights into selectivity and specificity., Macmaster R, Sedelnikova S, Baker PJ, Bolt EL, Lloyd RG, Rafferty JB, Nucleic Acids Res. 2006;34(19):5577-84. Epub 2006 Oct 5. PMID:17028102 Page seeded by OCA on Sun May 4 05:59:15 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2h8c.jpg|left|200px]]
- {{Structure
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-|GENE= rusA, rus ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h8c OCA], [http://www.ebi.ac.uk/pdbsum/2h8c PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2h8c RCSB]</span>
-}}
 '''Structure of RusA D70N in complex with DNA'''
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 [[Category: Single protein]]
 [[Category: Macmaster, R A.]]
-[[Category: protein-dna complex]]
+[[Category: Protein-dna complex]]
-[[Category: recombination]]
+[[Category: Recombination]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 05:59:15 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:26:54 2008''