2h6t: Difference between revisions

Revision as of 05:56, 4 May 2008

Secreted aspartic proteinase (Sap) 3 from Candida albicans complexed with pepstatin A

OverviewOverview

The family of secreted aspartic proteinases (Sap) encoded by 10 SAP genes is an important virulence factor during Candida albicans (C. albicans) infections. Antagonists to Saps could be envisioned to help prevent or treat candidosis in immunocompromised patients. The knowledge of several Sap structures is crucial for inhibitor design; only the structure of Sap2 is known. We report the 1.9 and 2.2 A resolution X-ray crystal structures of Sap3 in a stable complex with pepstatin A and in the absence of an inhibitor, shedding further light on the enzyme inhibitor binding. Inhibitor binding causes active site closure by the movement of a flap segment. Comparison of the structures of Sap3 and Sap2 identifies elements responsible for the specificity of each isoenzyme.

About this StructureAbout this Structure

2H6T is a Single protein structure of sequence from Candida albicans. Full crystallographic information is available from OCA.

ReferenceReference

The crystal structure of the secreted aspartic proteinase 3 from Candida albicans and its complex with pepstatin A., Borelli C, Ruge E, Schaller M, Monod M, Korting HC, Huber R, Maskos K, Proteins. 2007 Aug 15;68(3):738-48. PMID:17510964 Page seeded by OCA on Sun May 4 05:56:12 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2h6t.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2h6t |SIZE=350|CAPTION= <scene name='initialview01'>2h6t</scene>, resolution 1.900&Aring;
+The line below this paragraph, containing "STRUCTURE_2h6t", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=IHN:3-HYDROXY-4-ISOBUTYL-4-[AMINOCARBONYLETHYL(AMINO+CARBONYL-2-HYDROXY-5-METHYLHEXYL)TRI(AMINOCARBONYL+ISOBUTYL)]BUTANOIC+ACID'>IHN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Candidapepsin Candidapepsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.24 3.4.23.24] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= SAP3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5476 Candida albicans])
+-->
-|DOMAIN=
+{{STRUCTURE_2h6t|  PDB=2h6t  |  SCENE=  }}
-|RELATEDENTRY=[[2h6s|2H6S]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h6t OCA], [http://www.ebi.ac.uk/pdbsum/2h6t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2h6t RCSB]</span>
-}}
 '''Secreted aspartic proteinase (Sap) 3 from Candida albicans complexed with pepstatin A'''
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 [[Category: Maskos, K.]]
 [[Category: Ruge, E.]]
-[[Category: aspartic proteinase]]
+[[Category: Aspartic proteinase]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 05:56:12 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:26:14 2008''