2h4m: Difference between revisions

Revision as of 05:51, 4 May 2008

Karyopherin Beta2/Transportin-M9NLS

OverviewOverview

Karyopherinbeta (Kapbeta) proteins bind nuclear localization and export signals (NLSs and NESs) to mediate nucleocytoplasmic trafficking, a process regulated by Ran GTPase through its nucleotide cycle. Diversity and complexity of signals recognized by Kap betas have prevented prediction of new Kap beta substrates. The structure of Kap beta 2 (also known as Transportin) bound to one of its substrates, the NLS of hnRNP A1, that we report here explains the mechanism of substrate displacement by Ran GTPase. Further analyses reveal three rules for NLS recognition by Kap beta 2: NLSs are structurally disordered in free substrates, have overall basic character, and possess a central hydrophobic or basic motif followed by a C-terminal R/H/KX(2-5)PY consensus sequence. We demonstrate the predictive nature of these rules by identifying NLSs in seven previously known Kap beta 2 substrates and uncovering 81 new candidate substrates, confirming five experimentally. These studies define and validate a new NLS that could not be predicted by primary sequence analysis alone.

About this StructureAbout this Structure

2H4M is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Rules for nuclear localization sequence recognition by karyopherin beta 2., Lee BJ, Cansizoglu AE, Suel KE, Louis TH, Zhang Z, Chook YM, Cell. 2006 Aug 11;126(3):543-58. PMID:16901787 Page seeded by OCA on Sun May 4 05:51:38 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2h4m.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_2h4m", creates the "Structure Box" on the page.
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-|GENE= TNPO1, KPNB2, MIP1, TRN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), HNRPA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+-->
-|DOMAIN=
+{{STRUCTURE_2h4m|  PDB=2h4m  |  SCENE=  }}
-|RELATEDENTRY=[[1qbk|1QBK]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h4m OCA], [http://www.ebi.ac.uk/pdbsum/2h4m PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2h4m RCSB]</span>
-}}
 '''Karyopherin Beta2/Transportin-M9NLS'''
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 [[Category: Cansizoglu, A E.]]
 [[Category: Chook, Y M.]]
-[[Category: heat repeat]]
+[[Category: Heat repeat]]
-[[Category: nuclear import complex]]
+[[Category: Nuclear import complex]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 05:51:38 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:25:22 2008''