2h2y: Difference between revisions

Revision as of 05:48, 4 May 2008

Crystal structure of ubiquitin conjugating enzyme E2 from plasmodium falciparum

OverviewOverview

Parasites from the protozoan phylum Apicomplexa are responsible for diseases, such as malaria, toxoplasmosis and cryptosporidiosis, all of which have significantly higher rates of mortality and morbidity in economically underdeveloped regions of the world. Advances in vaccine development and drug discovery are urgently needed to control these diseases and can be facilitated by production of purified recombinant proteins from Apicomplexan genomes and determination of their 3D structures. To date, both heterologous expression and crystallization of Apicomplexan proteins have seen only limited success. In an effort to explore the effectiveness of producing and crystallizing proteins on a genome-scale using a standardized methodology, over 400 distinct Plasmodium falciparum target genes were chosen representing different cellular classes, along with select orthologues from four other Plasmodium species as well as Cryptosporidium parvum and Toxoplasma gondii. From a total of 1008 genes from the seven genomes, 304 (30.2%) produced purified soluble proteins and 97 (9.6%) crystallized, culminating in 36 crystal structures. These results demonstrate that, contrary to previous findings, a standardized platform using Escherichia coli can be effective for genome-scale production and crystallography of Apicomplexan proteins. Predictably, orthologous proteins from different Apicomplexan genomes behaved differently in expression, purification and crystallization, although the overall success rates of Plasmodium orthologues do not differ significantly. Their differences were effectively exploited to elevate the overall productivity to levels comparable to the most successful ongoing structural genomics projects: 229 of the 468 target genes produced purified soluble protein from one or more organisms, with 80 and 32 of the purified targets, respectively, leading to crystals and ultimately structures from one or more orthologues.

About this StructureAbout this Structure

2H2Y is a Single protein structure of sequence from Plasmodium falciparum. Full crystallographic information is available from OCA.

ReferenceReference

Genome-scale protein expression and structural biology of Plasmodium falciparum and related Apicomplexan organisms., Vedadi M, Lew J, Artz J, Amani M, Zhao Y, Dong A, Wasney GA, Gao M, Hills T, Brokx S, Qiu W, Sharma S, Diassiti A, Alam Z, Melone M, Mulichak A, Wernimont A, Bray J, Loppnau P, Plotnikova O, Newberry K, Sundararajan E, Houston S, Walker J, Tempel W, Bochkarev A, Kozieradzki I, Edwards A, Arrowsmith C, Roos D, Kain K, Hui R, Mol Biochem Parasitol. 2007 Jan;151(1):100-10. Epub 2006 Nov 13. PMID:17125854 Page seeded by OCA on Sun May 4 05:48:25 2008

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OCA

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 [[Image:2h2y.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2h2y |SIZE=350|CAPTION= <scene name='initialview01'>2h2y</scene>, resolution 2.8&Aring;
+The line below this paragraph, containing "STRUCTURE_2h2y", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= MAL13P1.227 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5833 Plasmodium falciparum])
+-->
-|DOMAIN=
+{{STRUCTURE_2h2y|  PDB=2h2y  |  SCENE=  }}
-|RELATEDENTRY=[[2fo3|2FO3]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h2y OCA], [http://www.ebi.ac.uk/pdbsum/2h2y PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2h2y RCSB]</span>
-}}
 '''Crystal structure of ubiquitin conjugating enzyme E2 from plasmodium falciparum'''
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 [[Category: Weigelt, J.]]
 [[Category: Zhao, Y.]]
-[[Category: sgc]]
+[[Category: Sgc]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
-[[Category: structural genomics consortium]]
+[[Category: Structural genomics consortium]]
-[[Category: unknown function]]
+[[Category: Unknown function]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 05:48:25 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:24:42 2008''