2h1x: Difference between revisions

Revision as of 05:46, 4 May 2008

Crystal structure of 5-hydroxyisourate Hydrolase (formerly known as TRP, Transthyretin Related Protein)

OverviewOverview

During early vertebrate evolution, a duplication event in the gene encoding 5-hydroxyisourate hydrolase (HIUase), a widely distributed enzyme of purine metabolism, gave rise to transthyretin (TTR), a thyroid hormone transporter. We report here on the crystal structure of zebra fish HIUase in two different crystal forms. Despite the phylogenetic distance, this structure compares well with those of newly characterized bacterial HIUases, especially with regard to catalytic regions, which are highly preserved. Comparison with TTR structure reveals a highly conserved scaffold, harbouring distinct functional sites located in the same regions of the two vertebrate proteins. Residues that are differentially conserved in HIUases compared to TTR map in putative catalytic regions occupying significant portions of the two halves of a central channel that transverses the whole TTR protein. The evolution of TTR has been accompanied by remarkable changes of the HIUase active sites that gave rise to a channel open at both ends, thus allowing free access to hormone molecules.

About this StructureAbout this Structure

2H1X is a Single protein structure of sequence from Danio rerio. Full crystallographic information is available from OCA.

ReferenceReference

Structure of zebra fish HIUase: insights into evolution of an enzyme to a hormone transporter., Zanotti G, Cendron L, Ramazzina I, Folli C, Percudani R, Berni R, J Mol Biol. 2006 Oct 13;363(1):1-9. Epub 2006 Aug 2. PMID:16952372 Page seeded by OCA on Sun May 4 05:46:39 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2h1x.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_2h1x", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydroxyisourate_hydrolase Hydroxyisourate hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.17 3.5.2.17] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= GeneID:558664 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7955 Danio rerio])
+-->
-|DOMAIN=
+{{STRUCTURE_2h1x|  PDB=2h1x  |  SCENE=  }}
-|RELATEDENTRY=[[1f41|1F41]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h1x OCA], [http://www.ebi.ac.uk/pdbsum/2h1x PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2h1x RCSB]</span>
-}}
 '''Crystal structure of 5-hydroxyisourate Hydrolase (formerly known as TRP, Transthyretin Related Protein)'''
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 [[Category: Zanotti, G.]]
 [[Category: 5-hydroxyisourate hydrolase]]
-[[Category: allantoin]]
+[[Category: Allantoin]]
-[[Category: trp]]
+[[Category: Trp]]
-[[Category: uric acid degradation]]
+[[Category: Uric acid degradation]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 05:46:39 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:24:24 2008''