2gnt: Difference between revisions

Revision as of 05:19, 4 May 2008

EDTA treated P. angolensis lectin (PAL) remetallized with calcium (1 hour treatment)

OverviewOverview

The interplay between metal binding, carbohydrate binding activity, stability and structure of the lectin from Pterocarpus angolensis was investigated. Removal of the metals leads to a more flexible form of the protein with significantly less conformational stability. Crystal structures of this metal-free form show significant structural rearrangements, although some structural features that allow the binding of sugars are retained. We propose that substitution of an asparagine residue at the start of the C-terminal beta-strand of the legume lectin monomer hinders the trans-isomerization of the cis-peptide bond upon demetallization and constitutes an intramolecular switch governing the isomer state of the non-proline bond and ultimately the lectin phenotype.

About this StructureAbout this Structure

2GNT is a Single protein structure of sequence from Pterocarpus angolensis. Full crystallographic information is available from OCA.

ReferenceReference

Interplay between metal binding and cis/trans isomerization in legume lectins: structural and thermodynamic study of P. angolensis lectin., Garcia-Pino A, Buts L, Wyns L, Loris R, J Mol Biol. 2006 Aug 4;361(1):153-67. Epub 2006 Jun 21. PMID:16824540 Page seeded by OCA on Sun May 4 05:19:12 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2gnt.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2gnt |SIZE=350|CAPTION= <scene name='initialview01'>2gnt</scene>, resolution 2.02&Aring;
+The line below this paragraph, containing "STRUCTURE_2gnt", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2gnt|  PDB=2gnt  |  SCENE=  }}
-|RELATEDENTRY=[[1ukg|1UKG]], [[2gme|2GME]], [[2gmm|2GMM]], [[2gmp|2GMP]], [[2gn3|2GN3]], [[2gnb|2GNB]], [[2gnm|2GNM]], [[2gnd|2GND]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gnt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gnt OCA], [http://www.ebi.ac.uk/pdbsum/2gnt PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gnt RCSB]</span>
-}}
 '''EDTA treated P. angolensis lectin (PAL) remetallized with calcium (1 hour treatment)'''
@@ Line 29: / Line 26: @@
 [[Category: Loris, R.]]
 [[Category: Wyns, L.]]
-[[Category: beta sandwich]]
+[[Category: Beta sandwich]]
-[[Category: legume lectin]]
+[[Category: Legume lectin]]
-[[Category: metal binding]]
+[[Category: Metal binding]]
-[[Category: pal]]
+[[Category: Pal]]
-[[Category: remetallization]]
+[[Category: Remetallization]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 05:19:12 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:19:09 2008''