2gg4: Difference between revisions

Revision as of 05:04, 4 May 2008

CP4 EPSP synthase (unliganded)

OverviewOverview

The engineering of transgenic crops resistant to the broad-spectrum herbicide glyphosate has greatly improved agricultural efficiency worldwide. Glyphosate-based herbicides, such as Roundup, target the shikimate pathway enzyme 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase, the functionality of which is absolutely required for the survival of plants. Roundup Ready plants carry the gene coding for a glyphosate-insensitive form of this enzyme, obtained from Agrobacterium sp. strain CP4. Once incorporated into the plant genome, the gene product, CP4 EPSP synthase, confers crop resistance to glyphosate. Although widely used, the molecular basis for this glyphosate-resistance has remained obscure. We generated a synthetic gene coding for CP4 EPSP synthase and characterized the enzyme using kinetics and crystallography. The CP4 enzyme has unexpected kinetic and structural properties that render it unique among the known EPSP synthases. Glyphosate binds to the CP4 EPSP synthase in a condensed, noninhibitory conformation. Glyphosate sensitivity can be restored through a single-site mutation in the active site (Ala-100-Gly), allowing glyphosate to bind in its extended, inhibitory conformation.

About this StructureAbout this Structure

2GG4 is a Single protein structure of sequence from Agrobacterium sp.. Full crystallographic information is available from OCA.

ReferenceReference

Molecular basis for the herbicide resistance of Roundup Ready crops., Funke T, Han H, Healy-Fried ML, Fischer M, Schonbrunn E, Proc Natl Acad Sci U S A. 2006 Aug 29;103(35):13010-5. Epub 2006 Aug 17. PMID:16916934 Page seeded by OCA on Sun May 4 05:04:09 2008

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OCA

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 [[Image:2gg4.gif|left|200px]]
- {{Structure
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] </span>
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-|GENE= aroA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=361 Agrobacterium sp.])
+-->
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-|RELATEDENTRY=[[1g6t|1G6T]], [[1g6s|1G6S]], [[1q36|1Q36]], [[1mi4|1MI4]], [[1rf5|1RF5]], [[1rf6|1RF6]], [[2gg6|2GG6]], [[2gga|2GGA]], [[2ggd|2GGD]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gg4 OCA], [http://www.ebi.ac.uk/pdbsum/2gg4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gg4 RCSB]</span>
-}}
 '''CP4 EPSP synthase (unliganded)'''
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 [[Category: Funke, T.]]
 [[Category: Schonbrunn, E.]]
-[[Category: inside-out alpha/beta barrel]]
+[[Category: Inside-out alpha/beta barrel]]
-[[Category: two domain structure]]
+[[Category: Two domain structure]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 05:04:09 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:16:09 2008''