2gdy: Difference between revisions

Revision as of 04:59, 4 May 2008

Solution structure of the B. brevis TycC3-PCP in A-state

OverviewOverview

Protein dynamics plays an important role in protein function. Many functionally important motions occur on the microsecond and low millisecond time scale and can be characterized by nuclear magnetic resonance relaxation experiments. We describe the different states of a peptidyl carrier protein (PCP) that play a crucial role in its function as a peptide shuttle in the nonribosomal peptide synthetases of the tyrocidine A system. Both apo-PCP (without the bound 4'-phosphopantetheine cofactor) and holo-PCP exist in two different stable conformations. We show that one of the apo conformations and one of the holo conformations are identical, whereas the two remaining conformations are only detectable by nuclear magnetic resonance spectroscopy in either the apo or holo form. We further demonstrate that this conformational diversity is an essential prerequisite for the directed movement of the 4'-PP cofactor and its interaction with externally acting proteins such as thioesterases and 4'-PP transferase.

About this StructureAbout this Structure

2GDY is a Single protein structure of sequence from Brevibacillus parabrevis. Full crystallographic information is available from OCA.

ReferenceReference

Conformational switches modulate protein interactions in peptide antibiotic synthetases., Koglin A, Mofid MR, Lohr F, Schafer B, Rogov VV, Blum MM, Mittag T, Marahiel MA, Bernhard F, Dotsch V, Science. 2006 Apr 14;312(5771):273-6. PMID:16614225 Page seeded by OCA on Sun May 4 04:59:52 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2gdy.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2gdy |SIZE=350|CAPTION= <scene name='initialview01'>2gdy</scene>
+The line below this paragraph, containing "STRUCTURE_2gdy", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= tycC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=54914 Brevibacillus parabrevis])
+-->
-|DOMAIN=
+{{STRUCTURE_2gdy|  PDB=2gdy  |  SCENE=  }}
-|RELATEDENTRY=[[2gdw|2GDW]], [[2gdx|2GDX]], [[2ge1|2GE1]], [[2ge0|2GE0]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gdy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gdy OCA], [http://www.ebi.ac.uk/pdbsum/2gdy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gdy RCSB]</span>
-}}
 '''Solution structure of the B. brevis TycC3-PCP in A-state'''
@@ Line 31: / Line 28: @@
 [[Category: Marahiel, M A.]]
 [[Category: Rogov, V V.]]
-[[Category: three-helix bundle]]
+[[Category: Three-helix bundle]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 04:59:52 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:15:21 2008''