2g7e: Difference between revisions

Revision as of 04:46, 4 May 2008

The 1.6 A crystal structure of Vibrio cholerae extracellular endonuclease I

OverviewOverview

The crystal structure of a periplasmic/extracellular endonuclease from Vibrio cholerae has been solved at low and at neutral pH. Crystals grown at pH 4.6 and 6.9 diffracted to 1.6 A (on BM01A at the ESRF) and 1.95 A (on a rotating-anode generator), respectively. The structures of the endonuclease were compared with the structure of a homologous enzyme in V. vulnificus. The structures of the V. cholerae enzyme at different pH values are essentially identical to each other and to the V. vulnificus enzyme. However, interesting features were observed in the solvent structures. Both V. cholerae structures reveal the presence of a chloride ion completely buried within the core of the protein, with the nearest solvent molecule approximately 7 A away. Magnesium, which is essential for catalysis, is present in the structure at neutral pH, but is absent at low pH, and may partly explain the inactivity of the enzyme at lower pH.

About this StructureAbout this Structure

2G7E is a Single protein structure of sequence from Vibrio cholerae. Full crystallographic information is available from OCA.

ReferenceReference

The structure of Vibrio cholerae extracellular endonuclease I reveals the presence of a buried chloride ion., Altermark B, Smalas AO, Willassen NP, Helland R, Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1387-91. Epub 2006, Oct 18. PMID:17057343 Page seeded by OCA on Sun May 4 04:46:29 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2g7e.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 2g7e |SIZE=350|CAPTION= <scene name='initialview01'>2g7e</scene>, resolution 1.600&Aring;
+The line below this paragraph, containing "STRUCTURE_2g7e", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribonuclease_I Deoxyribonuclease I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.1 3.1.21.1] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= EndA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae])
+-->
-|DOMAIN=
+{{STRUCTURE_2g7e|  PDB=2g7e  |  SCENE=  }}
-|RELATEDENTRY=[[2g7f|2G7F]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g7e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g7e OCA], [http://www.ebi.ac.uk/pdbsum/2g7e PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2g7e RCSB]</span>
-}}
 '''The 1.6 A crystal structure of Vibrio cholerae extracellular endonuclease I'''
@@ Line 30: / Line 27: @@
 [[Category: Smalaas, A O.]]
 [[Category: Willassen, N P.]]
-[[Category: enda]]
+[[Category: Enda]]
-[[Category: endonuclease i;buried chloride]]
+[[Category: Endonuclease i;buried chloride]]
-[[Category: vibrio cholerae nuclease]]
+[[Category: Vibrio cholerae nuclease]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 04:46:29 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:12:49 2008''