2g6y: Difference between revisions

Revision as of 04:45, 4 May 2008

Crystal structure of the novel green fluorescent protein from marine copepod Pontellina plumata

OverviewOverview

Since the cloning of Aequorea victoria green fluorescent protein (GFP) in 1992, a family of known GFP-like proteins has been growing rapidly. Today, it includes more than a hundred proteins with different spectral characteristics cloned from Cnidaria species. For some of these proteins, crystal structures have been solved, showing diversity in chromophore modifications and conformational states. However, we are still far from a complete understanding of the origin, functions and evolution of the GFP family. Novel proteins of the family were recently cloned from evolutionarily distant marine Copepoda species, phylum Arthropoda, demonstrating an extremely rapid generation of fluorescent signal. Here, we have generated a non-aggregating mutant of Copepoda fluorescent protein and solved its high-resolution crystal structure. It was found that the protein beta-barrel contains a pore, leading to the chromophore. Using site-directed mutagenesis, we showed that this feature is critical for the fast maturation of the chromophore.

About this StructureAbout this Structure

2G6Y is a Single protein structure of sequence from Pontellina plumata. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for the fast maturation of Arthropoda green fluorescent protein., Evdokimov AG, Pokross ME, Egorov NS, Zaraisky AG, Yampolsky IV, Merzlyak EM, Shkoporov AN, Sander I, Lukyanov KA, Chudakov DM, EMBO Rep. 2006 Oct;7(10):1006-12. Epub 2006 Aug 25. PMID:16936637 Page seeded by OCA on Sun May 4 04:45:33 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2g6y.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2g6y |SIZE=350|CAPTION= <scene name='initialview01'>2g6y</scene>, resolution 1.600&Aring;
+The line below this paragraph, containing "STRUCTURE_2g6y", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CQR:[(4Z)-2-(AMINOMETHYL)-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>CQR</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= ppluGFP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=239963 Pontellina plumata])
+-->
-|DOMAIN=
+{{STRUCTURE_2g6y|  PDB=2g6y  |  SCENE=  }}
-|RELATEDENTRY=[[2g6x|2G6X]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g6y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g6y OCA], [http://www.ebi.ac.uk/pdbsum/2g6y PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2g6y RCSB]</span>
-}}
 '''Crystal structure of the novel green fluorescent protein from marine copepod Pontellina plumata'''
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 [[Category: Evdokimov, A G.]]
 [[Category: Pokross, M E.]]
-[[Category: beta-can]]
+[[Category: Beta-can]]
-[[Category: green fluorescent protein]]
+[[Category: Green fluorescent protein]]
-[[Category: natural chromophore]]
+[[Category: Natural chromophore]]
-[[Category: rapid maturation]]
+[[Category: Rapid maturation]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 04:45:33 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:12:41 2008''