2g45: Difference between revisions

Revision as of 04:40, 4 May 2008

Co-crystal structure of znf ubp domain from the deubiquitinating enzyme isopeptidase T (isot) in complex with ubiquitin

OverviewOverview

Ubiquitin binding proteins regulate the stability, function, and/or localization of ubiquitinated proteins. Here we report the crystal structures of the zinc-finger ubiquitin binding domain (ZnF UBP) from the deubiquitinating enzyme isopeptidase T (IsoT, or USP5) alone and in complex with ubiquitin. Unlike other ubiquitin binding domains, this domain contains a deep binding pocket where the C-terminal diglycine motif of ubiquitin is inserted, thus explaining the specificity of IsoT for an unmodified C terminus on the proximal subunit of polyubiquitin. Mutations in the domain demonstrate that it is required for optimal catalytic activation of IsoT. This domain is present in several other protein families, and the ZnF UBP domain from an E3 ligase also requires the C terminus of ubiquitin for binding. These data suggest that binding the ubiquitin C terminus may be necessary for the function of other proteins.

About this StructureAbout this Structure

2G45 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

The ubiquitin binding domain ZnF UBP recognizes the C-terminal diglycine motif of unanchored ubiquitin., Reyes-Turcu FE, Horton JR, Mullally JE, Heroux A, Cheng X, Wilkinson KD, Cell. 2006 Mar 24;124(6):1197-208. PMID:16564012 Page seeded by OCA on Sun May 4 04:40:12 2008

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OCA

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 [[Image:2g45.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2g45 |SIZE=350|CAPTION= <scene name='initialview01'>2g45</scene>, resolution 1.99&Aring;
+The line below this paragraph, containing "STRUCTURE_2g45", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin_thiolesterase Ubiquitin thiolesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.2.15 3.1.2.15] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= USP5, ISOT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+-->
-|DOMAIN=
+{{STRUCTURE_2g45|  PDB=2g45  |  SCENE=  }}
-|RELATEDENTRY=[[2g43|2G43]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g45 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g45 OCA], [http://www.ebi.ac.uk/pdbsum/2g45 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2g45 RCSB]</span>
-}}
 '''Co-crystal structure of znf ubp domain from the deubiquitinating enzyme isopeptidase T (isot) in complex with ubiquitin'''
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 [[Category: Reyes-Turcu, F E.]]
 [[Category: Wilkinson, K D.]]
-[[Category: deubiquitinating enzyme]]
+[[Category: Deubiquitinating enzyme]]
-[[Category: ubiquitin]]
+[[Category: Ubiquitin]]
-[[Category: zinc finger]]
+[[Category: Zinc finger]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 04:40:12 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:11:30 2008''