2g3q: Difference between revisions

Revision as of 04:39, 4 May 2008

Solution Structure of Ede1 UBA-ubiquitin complex

OverviewOverview

Monoubiquitination is a general mechanism for downregulating the activity of cell surface receptors by consigning these proteins for lysosome-mediated degradation through the endocytic pathway. The yeast Ede1 protein functions at the internalization step of endocytosis and binds monoubiquitinated proteins through a ubiquitin associated (UBA) domain. UBA domains are found in a broad range of cellular proteins but previous studies have suggested that the mode of ubiquitin recognition might not be universally conserved. Here we present the solution structure of the Ede1 UBA domain in complex with monoubiquitin. The Ede1 UBA domain forms a three-helix bundle structure and binds ubiquitin through a largely hydrophobic surface in a manner reminiscent of the Dsk2 UBA and the remotely homologous Cue2 CUE domains, for which high-resolution structures have been described. However, the interaction is dissimilar to the molecular models proposed for the hHR23A UBA domains bound to either monoubiquitin or Lys48-linked diubiquitin. Our mutational analyses of the Ede1 UBA domain-ubiquitin interaction reveal several key affinity determinants and, unexpectedly, a negative affinity determinant in the wild-type Ede1 protein, implying that high-affinity interactions may not be the sole criterion for optimal function of monoubiquitin-binding endocytic proteins.

About this StructureAbout this Structure

2G3Q is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for monoubiquitin recognition by the Ede1 UBA domain., Swanson KA, Hicke L, Radhakrishnan I, J Mol Biol. 2006 May 5;358(3):713-24. Epub 2006 Mar 9. PMID:16563434 Page seeded by OCA on Sun May 4 04:39:23 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2g3q.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2g3q |SIZE=350|CAPTION= <scene name='initialview01'>2g3q</scene>
+The line below this paragraph, containing "STRUCTURE_2g3q", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= Ede1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
+-->
-|DOMAIN=
+{{STRUCTURE_2g3q|  PDB=2g3q  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g3q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g3q OCA], [http://www.ebi.ac.uk/pdbsum/2g3q PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2g3q RCSB]</span>
-}}
 '''Solution Structure of Ede1 UBA-ubiquitin complex'''
@@ Line 28: / Line 25: @@
 [[Category: Radhakrishnan, I.]]
 [[Category: Swanson, K A.]]
-[[Category: endocytosis]]
+[[Category: Endocytosis]]
-[[Category: monoubiquitin signaling]]
+[[Category: Monoubiquitin signaling]]
-[[Category: solution structure]]
+[[Category: Solution structure]]
-[[Category: uba domain]]
+[[Category: Uba domain]]
-[[Category: ubiquitin-binding motif]]
+[[Category: Ubiquitin-binding motif]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 04:39:23 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:11:21 2008''