2fub: Difference between revisions

Revision as of 04:19, 4 May 2008

Crystal structure of urate oxidase at 140 MPa

OverviewOverview

We report the three-dimensional structure determined by high-pressure macromolecular crystallography (HPMX) of a 135-kDa homo-tetrameric enzyme, urate oxidase from Aspergillus flavus complexed with its potent inhibitor 8-azaxanthin. Urate oxidase crystals are quite sensitive to pressure, as three-dimensional order is lost at about 180 MPa. A highly complete 2.3 A resolution data set was collected at 140 MPa, close to the critical pressure. Crystal structures at atmospheric pressure and at high pressure were refined in the orthorhombic space group I222 with final crystallographic R factors 14.1% and 16.1%, respectively. The effect of pressure on temperature factors, ordered water molecules, hydrogen bond lengths, contacts, buried surface areas as well as cavity volume was investigated. Results suggest that the onset of disruption of the tetrameric assembly by pressure has been captured in the crystalline state.

About this StructureAbout this Structure

2FUB is a Single protein structure of sequence from Aspergillus flavus. Full crystallographic information is available from OCA.

ReferenceReference

High pressure macromolecular crystallography: the 140-MPa crystal structure at 2.3 A resolution of urate oxidase, a 135-kDa tetrameric assembly., Colloc'h N, Girard E, Dhaussy AC, Kahn R, Ascone I, Mezouar M, Fourme R, Biochim Biophys Acta. 2006 Mar;1764(3):391-7. Epub 2006 Jan 26. PMID:16478683 Page seeded by OCA on Sun May 4 04:19:08 2008

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OCA

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 [[Image:2fub.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2fub |SIZE=350|CAPTION= <scene name='initialview01'>2fub</scene>, resolution 2.30&Aring;
+The line below this paragraph, containing "STRUCTURE_2fub", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=AZA:8-AZAXANTHINE'>AZA</scene>, <scene name='pdbligand=CYS:CYSTEINE'>CYS</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Urate_oxidase Urate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.3 1.7.3.3] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2fub|  PDB=2fub  |  SCENE=  }}
-|RELATEDENTRY=[[1r51|1R51]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fub OCA], [http://www.ebi.ac.uk/pdbsum/2fub PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fub RCSB]</span>
-}}
 '''Crystal structure of urate oxidase at 140 MPa'''
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 [[Category: Fourme, R.]]
 [[Category: Girard, E.]]
-[[Category: h, N Colloc.]]
+[[Category: H, N Colloc.]]
-[[Category: high pressure]]
+[[Category: High pressure]]
-[[Category: tetramer]]
+[[Category: Tetramer]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 04:19:08 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:07:45 2008''