2fsg: Difference between revisions

Revision as of 04:15, 4 May 2008

Complex SecA:ATP from Escherichia coli

OverviewOverview

SecA is the preprotein translocase ATPase subunit and a superfamily 2 (SF2) RNA helicase. Here we present the 2 A crystal structures of the Escherichia coli SecA homodimer in the apo form and in complex with ATP, ADP and adenosine 5'-[beta,gamma-imido]triphosphate (AMP-PNP). Each monomer contains the SF2 ATPase core (DEAD motor) built of two domains (nucleotide binding domain, NBD and intramolecular regulator of ATPase 2, IRA2), the preprotein binding domain (PBD), which is inserted in NBD and a carboxy-terminal domain (C-domain) linked to IRA2. The structures of the nucleotide complexes of SecA identify an interfacial nucleotide-binding cleft located between the two DEAD motor domains and residues critical for ATP catalysis. The dimer comprises two virtually identical protomers associating in an antiparallel fashion. Dimerization is mediated solely through extensive contacts of the DEAD motor domains leaving the C-domain facing outwards from the dimerization core. This dimerization mode explains the effect of functionally important mutations and is completely different from the dimerization models proposed for other SecA structures. The repercussion of these findings on translocase assembly and catalysis is discussed.

About this StructureAbout this Structure

2FSG is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Structure of dimeric SecA, the Escherichia coli preprotein translocase motor., Papanikolau Y, Papadovasilaki M, Ravelli RB, McCarthy AA, Cusack S, Economou A, Petratos K, J Mol Biol. 2007 Mar 9;366(5):1545-57. Epub 2006 Dec 23. PMID:17229438 Page seeded by OCA on Sun May 4 04:15:33 2008

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OCA

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 [[Image:2fsg.jpg|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_2fsg", creates the "Structure Box" on the page.
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-|GENE= secA, azi, pea, prlD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+-->
-|DOMAIN=
+{{STRUCTURE_2fsg|  PDB=2fsg  |  SCENE=  }}
-|RELATEDENTRY=[[2fsf|2FSF]], [[2fsh|2FSH]], [[2fsi|2FSI]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fsg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fsg OCA], [http://www.ebi.ac.uk/pdbsum/2fsg PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fsg RCSB]</span>
-}}
 '''Complex SecA:ATP from Escherichia coli'''
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 [[Category: Papanikolau, Y.]]
 [[Category: Petratos, K.]]
-[[Category: atpase]]
+[[Category: Atpase]]
-[[Category: dna-rna helicase]]
+[[Category: Dna-rna helicase]]
-[[Category: protein translocation]]
+[[Category: Protein translocation]]
-[[Category: seca]]
+[[Category: Seca]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 04:15:33 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:07:04 2008''