2fpg: Difference between revisions

Revision as of 04:10, 4 May 2008

Crystal structure of pig GTP-specific succinyl-CoA synthetase in complex with GDP

OverviewOverview

Two isoforms of succinyl-CoA synthetase exist in mammals, one specific for ATP and the other for GTP. The GTP-specific form of pig succinyl-CoA synthetase has been crystallized in the presence of GTP and the structure determined to 2.1 A resolution. GTP is bound in the ATP-grasp domain, where interactions of the guanine base with a glutamine residue (Gln-20beta) and with backbone atoms provide the specificity. The gamma-phosphate interacts with the side chain of an arginine residue (Arg-54beta) and with backbone amide nitrogen atoms, leading to tight interactions between the gamma-phosphate and the protein. This contrasts with the structures of ATP bound to other members of the family of ATP-grasp proteins where the gamma-phosphate is exposed, free to react with the other substrate. To test if GDP would interact with GTP-specific succinyl-CoA synthetase in the same way that ADP interacts with other members of the family of ATP-grasp proteins, the structure of GDP bound to GTP-specific succinyl-CoA synthetase was also determined. A comparison of the conformations of GTP and GDP shows that the bases adopt the same position but that changes in conformation of the ribose moieties and the alpha- and beta-phosphates allow the gamma-phosphate to interact with the arginine residue and amide nitrogen atoms in GTP, while the beta-phosphate interacts with these residues in GDP. The complex of GTP with succinyl-CoA synthetase shows that the enzyme is able to protect GTP from hydrolysis when the active-site histidine residue is not in position to be phosphorylated.

About this StructureAbout this Structure

2FPG is a Protein complex structure of sequences from Sus scrofa. Full crystallographic information is available from OCA.

ReferenceReference

Interactions of GTP with the ATP-grasp domain of GTP-specific succinyl-CoA synthetase., Fraser ME, Hayakawa K, Hume MS, Ryan DG, Brownie ER, J Biol Chem. 2006 Apr 21;281(16):11058-65. Epub 2006 Feb 15. PMID:16481318 Page seeded by OCA on Sun May 4 04:10:01 2008

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OCA

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 [[Image:2fpg.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2fpg |SIZE=350|CAPTION= <scene name='initialview01'>2fpg</scene>, resolution 2.960&Aring;
+The line below this paragraph, containing "STRUCTURE_2fpg", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=GDP:GUANOSINE-5&#39;-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Succinate--CoA_ligase_(GDP-forming) Succinate--CoA ligase (GDP-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.2.1.4 6.2.1.4] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= SUCLG1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9823 Sus scrofa]), SUCLG2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9823 Sus scrofa])
+-->
-|DOMAIN=
+{{STRUCTURE_2fpg|  PDB=2fpg  |  SCENE=  }}
-|RELATEDENTRY=[[1euc|1EUC]], [[1eud|1EUD]], [[2fp4|2FP4]], [[2fpi|2FPI]], [[2fpp|2FPP]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fpg OCA], [http://www.ebi.ac.uk/pdbsum/2fpg PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fpg RCSB]</span>
-}}
 '''Crystal structure of pig GTP-specific succinyl-CoA synthetase in complex with GDP'''
@@ Line 24: / Line 21: @@
 Interactions of GTP with the ATP-grasp domain of GTP-specific succinyl-CoA synthetase., Fraser ME, Hayakawa K, Hume MS, Ryan DG, Brownie ER, J Biol Chem. 2006 Apr 21;281(16):11058-65. Epub 2006 Feb 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16481318 16481318]
 [[Category: Protein complex]]
-[[Category: Succinate--CoA ligase (GDP-forming)]]
 [[Category: Sus scrofa]]
 [[Category: Fraser, M E.]]
-[[Category: active site histidine residue]]
+[[Category: Active site histidine residue]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 04:10:01 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:05:58 2008''