2fp1: Difference between revisions

Revision as of 04:09, 4 May 2008

Secreted Chorismate Mutase from Mycobacterium tuberculosis

OverviewOverview

The presence of exported chorismate mutases produced by certain organisms such as Mycobacterium tuberculosis has been shown to correlate with their pathogenicity. As such, these proteins comprise a new group of promising selective drug targets. Here, we report the high-resolution crystal structure of the secreted dimeric chorismate mutase from M. tuberculosis (*MtCM; encoded by Rv1885c), which represents the first 3D-structure of a member of this chorismate mutase family, termed the AroQ(gamma) subclass. Structures are presented both for the unliganded enzyme and for a complex with a transition state analog. The protomer fold resembles the structurally characterized (dimeric) Escherichia coli chorismate mutase domain, but exhibits a new topology, with helix H4 of *MtCM carrying the catalytic site residue missing in the shortened helix H1. Furthermore, the structure of each *MtCM protomer is significantly more compact and only harbors one active site pocket, which is formed entirely by one polypeptide chain. Apart from the structural model, we present evidence as to how the substrate may enter the active site.

About this StructureAbout this Structure

2FP1 is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

ReferenceReference

1.6 A crystal structure of the secreted chorismate mutase from Mycobacterium tuberculosis: novel fold topology revealed., Okvist M, Dey R, Sasso S, Grahn E, Kast P, Krengel U, J Mol Biol. 2006 Apr 14;357(5):1483-99. Epub 2006 Feb 6. PMID:16499927 Page seeded by OCA on Sun May 4 04:09:24 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:2fp1.gif|left|200px]]
- {{Structure
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-|PDB= 2fp1 |SIZE=350|CAPTION= <scene name='initialview01'>2fp1</scene>, resolution 1.55&Aring;
+The line below this paragraph, containing "STRUCTURE_2fp1", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=PB:LEAD+(II)+ION'>PB</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Chorismate_mutase Chorismate mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.5 5.4.99.5] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
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-|RELATEDENTRY=[[2fp2|2FP2]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fp1 OCA], [http://www.ebi.ac.uk/pdbsum/2fp1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fp1 RCSB]</span>
-}}
 '''Secreted Chorismate Mutase from Mycobacterium tuberculosis'''
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 [[Category: Okvist, M.]]
 [[Category: Sasso, S.]]
-[[Category: alpha-helical]]
+[[Category: Alpha-helical]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 04:09:24 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:05:49 2008''