2fll: Difference between revisions

Revision as of 04:02, 4 May 2008

Ternary complex of human DNA polymerase iota with DNA and dTTP

OverviewOverview

Substrate-induced conformational change of the protein is the linchpin of enzymatic reactions. Replicative DNA polymerases, for example, convert from an open to a closed conformation in response to dNTP binding. Human DNA polymerase-iota (hPoliota), a member of the Y family of DNA polymerases, differs strikingly from other polymerases in its much higher proficiency and fidelity for nucleotide incorporation opposite template purines than opposite template pyrimidines. We present here a crystallographic analysis of hPoliota binary complexes, which together with the ternary complexes show that, contrary to replicative DNA polymerases, the DNA, and not the polymerase, undergoes the primary substrate-induced conformational change. The incoming dNTP "pushes" templates A and G from the anti to the syn conformation dictated by a rigid hPoliota active site. Together, the structures posit a mechanism for template selection wherein dNTP binding induces a conformational switch in template purines for productive Hoogsteen base pairing.

About this StructureAbout this Structure

2FLL is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

An incoming nucleotide imposes an anti to syn conformational change on the templating purine in the human DNA polymerase-iota active site., Nair DT, Johnson RE, Prakash L, Prakash S, Aggarwal AK, Structure. 2006 Apr;14(4):749-55. PMID:16615915 Page seeded by OCA on Sun May 4 04:02:15 2008

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OCA

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 [[Image:2fll.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2fll |SIZE=350|CAPTION= <scene name='initialview01'>2fll</scene>, resolution 2.60&Aring;
+The line below this paragraph, containing "STRUCTURE_2fll", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DOC:2&#39;,3&#39;-DIDEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DOC</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5&#39;-TRIPHOSPHATE'>TTP</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= POLI, RAD30B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+-->
-|DOMAIN=
+{{STRUCTURE_2fll|  PDB=2fll  |  SCENE=  }}
-|RELATEDENTRY=[[1t3n|1T3N]], [[2azl|2AZL]], [[2fln|2FLN]], [[2flp|2FLP]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fll FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fll OCA], [http://www.ebi.ac.uk/pdbsum/2fll PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fll RCSB]</span>
-}}
 '''Ternary complex of human DNA polymerase iota with DNA and dTTP'''
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 [[Category: Prakash, L.]]
 [[Category: Prakash, S.]]
-[[Category: dna polymerase]]
+[[Category: Dna polymerase]]
-[[Category: lesion bypass]]
+[[Category: Lesion bypass]]
-[[Category: p6522]]
+[[Category: P6522]]
-[[Category: ternary complex]]
+[[Category: Ternary complex]]
-[[Category: y-family]]
+[[Category: Y-family]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 04:02:15 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:04:33 2008''