2fl1: Difference between revisions

Revision as of 04:01, 4 May 2008

Crystal structure of red fluorescent protein from Zoanthus, zRFP574, at 2.4A resolution

OverviewOverview

The three-dimensional structure of the red fluorescent protein (RFP) zRFP574 from the button polyp Zoanthus sp. (two dimers per asymmetric unit, 231 x 4 amino acids) has been determined at 2.4 A resolution in space group C222(1). The crystal structure, refined to a crystallographic R factor of 0.203 (R(free) = 0.249), adopts the beta-barrel fold composed of 11 strands similar to that of the yellow fluorescent protein zYFP538. The zRFP574 chromophore, originating from the protein sequence Asp66-Tyr67-Gly68, has a two-ring structure typical of GFP-like proteins. The bond geometry of residue 66 shows the strong tendency of the corresponding C(alpha) atom to sp(2) hybridization as a consequence of N-acylimine bond formation. The zRFP574 chromophore contains the 65-66 cis-peptide bond characteristic of red fluorescent proteins. The chromophore phenolic ring adopts a cis conformation coplanar with the imidazolinone ring. The crystallographic study has revealed an unexpected chemical feature of the internal chromophore. A decarboxylated side chain of the chromophore-forming residue Asp66 has been observed in the structure. This additional post-translational modification is likely to play a key role in the bathochromic shift of the zRFP574 spectrum.

About this StructureAbout this Structure

2FL1 is a Single protein structure of sequence from Zoanthus sp.. Full crystallographic information is available from OCA.

ReferenceReference

Structure of a red fluorescent protein from Zoanthus, zRFP574, reveals a novel chromophore., Pletneva N, Pletnev S, Tikhonova T, Popov V, Martynov V, Pletnev V, Acta Crystallogr D Biol Crystallogr. 2006 May;62(Pt 5):527-32. Epub 2006, Apr 19. PMID:16627946 Page seeded by OCA on Sun May 4 04:00:59 2008

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OCA

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 [[Image:2fl1.jpg|left|200px]]
- {{Structure
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-}}
 '''Crystal structure of red fluorescent protein from Zoanthus, zRFP574, at 2.4A resolution'''
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 [[Category: Popov, B.]]
 [[Category: Tikhonova, T.]]
-[[Category: beta barrel]]
+[[Category: Beta barrel]]
-[[Category: beta-can fold]]
+[[Category: Beta-can fold]]
-[[Category: button polyp]]
+[[Category: Button polyp]]
-[[Category: chromophore]]
+[[Category: Chromophore]]
-[[Category: crystal structure]]
+[[Category: Crystal structure]]
-[[Category: emission maximum 574nm]]
+[[Category: Emission maximum 574nm]]
-[[Category: fluorescent marker]]
+[[Category: Fluorescent marker]]
-[[Category: intersubunit interface]]
+[[Category: Intersubunit interface]]
-[[Category: red fluorescent protein]]
+[[Category: Red fluorescent protein]]
-[[Category: tightly packed tetramer]]
+[[Category: Tightly packed tetramer]]
-[[Category: zoanthus sp.]]
+[[Category: Zoanthus sp.]]
-[[Category: zrfp574]]
+[[Category: Zrfp574]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 04:00:59 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:04:12 2008''