2fff: Difference between revisions

Revision as of 03:49, 4 May 2008

Open Form of a Class A Transpeptidase Domain

OverviewOverview

The class A PBP1b from Streptococcus pneumoniae is responsible for glycosyltransferase and transpeptidase (TP) reactions, forming the peptidoglycan of the bacterial cell wall. The enzyme has been produced in a stable, soluble form and undergoes time-dependent proteolysis to leave an intact TP domain. Crystals of this TP domain were obtained, diffracting to 2.2 A resolution, and the structure was solved by using molecular replacement. Analysis of the structure revealed an "open" active site, with important conformational differences to the previously determined "closed" apoenzyme. The active-site nucleophile, Ser460, is in an orientation that allows for acylation by beta-lactams. Consistent with the productive conformation of the conserved active-site catalytic residues, adjacent loops show only minor deviation from those of known acyl-enzyme structures. These findings are discussed in the context of enzyme functionality and the possible conformational sampling of PBP1b between active and inactive states.

About this StructureAbout this Structure

2FFF is a Protein complex structure of sequences from Streptococcus pneumoniae. Full crystallographic information is available from OCA.

ReferenceReference

Structural analysis of an "open" form of PBP1B from Streptococcus pneumoniae., Lovering AL, De Castro L, Lim D, Strynadka NC, Protein Sci. 2006 Jul;15(7):1701-9. Epub 2006 Jun 2. PMID:16751607 Page seeded by OCA on Sun May 4 03:49:51 2008

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OCA

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 [[Image:2fff.gif|left|200px]]
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 '''Open Form of a Class A Transpeptidase Domain'''
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 [[Category: Lovering, A L.]]
 [[Category: Strynadka, N C.J.]]
-[[Category: transpeptidase fold]]
+[[Category: Transpeptidase fold]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 03:49:51 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:02:03 2008''