2feu: Difference between revisions

Revision as of 03:48, 4 May 2008

P450CAM from Pseudomonas putida reconstituted with manganic protoporphyrin IX

OverviewOverview

The oxidative prowess of the P450 cytochromes in physiological reactions is attributed to the production of a high-valent iron-oxo complex, or Compound I intermediate, in the reaction cycle. Despite many years of study, however, the full electronic description of this fleeting intermediate still remains an active area of study. In this manuscript, the current status of the isolation and characterization of the P450 oxo-Fe(IV) is examined and compared to analogous states in related heme enzymes. In addition, the utilization of cofactor exchange to stabilize high-valent oxo-states in the P450 is addressed. Structural and spectroscopic studies on manganese reconstituted P450, and its corresponding oxo-complex, are presented.

About this StructureAbout this Structure

2FEU is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.

ReferenceReference

The status of high-valent metal oxo complexes in the P450 cytochromes., Makris TM, von Koenig K, Schlichting I, Sligar SG, J Inorg Biochem. 2006 Apr;100(4):507-18. Epub 2006 Feb 28. PMID:16510191 Page seeded by OCA on Sun May 4 03:48:49 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2feu.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2feu |SIZE=350|CAPTION= <scene name='initialview01'>2feu</scene>, resolution 1.70&Aring;
+The line below this paragraph, containing "STRUCTURE_2feu", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CAM:CAMPHOR'>CAM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MNR:PROTOPORPHYRIN+IX+CONTAINING+MN'>MNR</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Camphor_5-monooxygenase Camphor 5-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.15.1 1.14.15.1] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= camC, cyp101 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 Pseudomonas putida])
+-->
-|DOMAIN=
+{{STRUCTURE_2feu|  PDB=2feu  |  SCENE=  }}
-|RELATEDENTRY=[[2fe6|2FE6]], [[2fer|2FER]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2feu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2feu OCA], [http://www.ebi.ac.uk/pdbsum/2feu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2feu RCSB]</span>
-}}
 '''P450CAM from Pseudomonas putida reconstituted with manganic protoporphyrin IX'''
@@ Line 30: / Line 27: @@
 [[Category: Schlichting, I.]]
 [[Category: Sligar, S G.]]
-[[Category: heme]]
+[[Category: Heme]]
-[[Category: manganic]]
+[[Category: Manganic]]
-[[Category: mono-oxygenase]]
+[[Category: Mono-oxygenase]]
-[[Category: substrate-bound]]
+[[Category: Substrate-bound]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 03:48:49 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:01:50 2008''