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'''Solution structure of G-quadruplex formed in the human Bcl-2 promoter''' | '''Solution structure of G-quadruplex formed in the human Bcl-2 promoter''' | ||
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==About this Structure== | ==About this Structure== | ||
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8U OCA]. | |||
==Reference== | ==Reference== | ||
NMR solution structure of the major G-quadruplex structure formed in the human BCL2 promoter region., Dai J, Chen D, Jones RA, Hurley LH, Yang D, Nucleic Acids Res. 2006;34(18):5133-44. Epub 2006 Sep 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16998187 16998187] | NMR solution structure of the major G-quadruplex structure formed in the human BCL2 promoter region., Dai J, Chen D, Jones RA, Hurley LH, Yang D, Nucleic Acids Res. 2006;34(18):5133-44. Epub 2006 Sep 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16998187 16998187] | ||
[[Category: Carver, M.]] | [[Category: Carver, M.]] | ||
[[Category: Chen, D.]] | [[Category: Chen, D.]] | ||
[[Category: Dai, J.]] | [[Category: Dai, J.]] | ||
[[Category: Yang, D.]] | [[Category: Yang, D.]] | ||
[[Category: | [[Category: Bcl-2 promoter]] | ||
[[Category: | [[Category: Dna]] | ||
[[Category: | [[Category: G-quadruplex]] | ||
[[Category: | [[Category: Nmr]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:36:09 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 03:36, 4 May 2008
Solution structure of G-quadruplex formed in the human Bcl-2 promoter
OverviewOverview
BCL2 protein functions as an inhibitor of cell apoptosis and has been found to be aberrantly expressed in a wide range of human diseases. A highly GC-rich region upstream of the P1 promoter plays an important role in the transcriptional regulation of BCL2. Here we report the NMR solution structure of the major intramolecular G-quadruplex formed on the G-rich strand of this region in K+ solution. This well-defined mixed parallel/antiparallel-stranded G-quadruplex structure contains three G-tetrads of mixed G-arrangements, which are connected with two lateral loops and one side loop, and four grooves of different widths. The three loops interact with the core G-tetrads in a specific way that defines and stabilizes the overall G-quadruplex structure. The loop conformations are in accord with the experimental mutation and footprinting data. The first 3-nt loop adopts a lateral loop conformation and appears to determine the overall folding of the BCL2 G-quadruplex. The third 1-nt double-chain-reversal loop defines another example of a stable parallel-stranded structural motif using the G3NG3 sequence. Significantly, the distinct major BCL2 promoter G-quadruplex structure suggests that it can be specifically involved in gene modulation and can be an attractive target for pathway-specific drug design.
About this StructureAbout this Structure
Full crystallographic information is available from OCA.
ReferenceReference
NMR solution structure of the major G-quadruplex structure formed in the human BCL2 promoter region., Dai J, Chen D, Jones RA, Hurley LH, Yang D, Nucleic Acids Res. 2006;34(18):5133-44. Epub 2006 Sep 22. PMID:16998187 Page seeded by OCA on Sun May 4 03:36:09 2008